The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 5µl for 106 cells. ab67435-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionCell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.
Tissue specificityExpressed in B cell lines and T lymphocytes.
Sequence similaritiesBelongs to the selectin/LECAM family. Contains 1 C-type lectin domain. Contains 1 EGF-like domain. Contains 2 Sushi (CCP/SCR) domains.
Staining of normal Human peripheral blood cells with PE-Cy5 Mouse IgG1 ? Isotype Control (open histogram) or ab95540 (filled histogram). Cells in the lymphocyte gate were used for analysis.
References for Anti-CD62L antibody [DREG-56] (PE/Cy5®) (ab95540)
This product has been referenced in:
Jutila MA et al. L-selectin serves as an E-selectin ligand on cultured human T lymphoblasts. J Immunol169:1768-73 (2002).
Read more (PubMed: 12165498) »
Kishimoto TK et al. Identification of a human peripheral lymph node homing receptor: a rapidly down-regulated adhesion molecule. Proc Natl Acad Sci U S A87:2244-8 (1990).
WB, Flow Cyt
Read more (PubMed: 2179952) »