FITC Anti-CD62L antibody [FITC.FMC46] (ab222)
Key features and details
- FITC Mouse monoclonal [FITC.FMC46] to CD62L
- Suitable for: Flow Cyt
- Reacts with: Human, Monkey
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2b
Overview
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Product name
FITC Anti-CD62L antibody [FITC.FMC46]
See all CD62L primary antibodies -
Description
FITC Mouse monoclonal [FITC.FMC46] to CD62L -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
FITC.FMC46 recognises the human CD62L cell surface antigen, a 150 kD glycoprotein also known as L-selectin. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human, Monkey -
Immunogen
Tissue, cells or virus corresponding to Human CD62L. PHA stimulated lymphoblasts
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General notes
Conjugated to Fluorescein Isothiocyanite Isomer 1 (FITC). FITC : Protein (molar ratio): 5.0 : 1.0.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified IgG prepared by affinity chromotography on protein G from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
FITC.FMC46 -
Myeloma
NS1 -
Isotype
IgG2b -
Light chain type
unknown -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use 10µl for 106 cells.
Use 10 µl to label 106 cells or 100 µl whole blood. ab91368 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Notes |
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Flow Cyt
Use 10µl for 106 cells. Use 10 µl to label 106 cells or 100 µl whole blood. ab91368 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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Function
Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia. -
Tissue specificity
Expressed in B cell lines and T lymphocytes. -
Sequence similarities
Belongs to the selectin/LECAM family.
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 2 Sushi (CCP/SCR) domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6402 Human
- Omim: 153240 Human
- SwissProt: P14151 Human
- Unigene: 728756 Human
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Alternative names
- A.11 antibody
- AI528707 antibody
- CD62 antigen ligand antibody
see all
Images
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ab183 staining CD62L (FITC) in human peripheral blood granulocytes by Flow Cytometry analysis.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab222 has been referenced in 1 publication.
- Lin HT et al. Characterization of pancreatic stem cells derived from adult human pancreas ducts by fluorescence activated cell sorting. World J Gastroenterol 12:4529-35 (2006). PubMed: 16874866