Mouse monoclonal [LT-TD180] to CD62L (Phycoerythrin)
Phycoerythrin. Ex: 488nm, Em: 575nm
Reacts with CD62L (L selectin), a 74-95 kDa single chain type I
glycoprotein expressed on most peripheral blood B lymphocytes, T lymphocytes, monocytes and granulocytes; it is also present on a subset of NK cells and certain hematopoietic malignant cells.
The purified antibody (protein A (G) affinity chromatography before conjugation) is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 20µl reagent / 100µl of whole blood or 106 cells in a suspension. (sufficient for 100 tests).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.
Expressed in B cell lines and T lymphocytes.
Belongs to the selectin/LECAM family. Contains 1 C-type lectin domain. Contains 1 EGF-like domain. Contains 2 Sushi (CCP/SCR) domains.
Linnebacher M et al. Identification of an MSI-H tumor-specific cytotoxic T cell epitope generated by the (-1) frame of U79260(FTO). J Biomed Biotechnol2010:841451 (2010).
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