Mouse monoclonal [LT-TD180] to CD62L (Phycoerythrin)
ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
SpecificityReacts with CD62L (L selectin), a 74-95 kDa single chain type I
glycoprotein expressed on most peripheral blood B lymphocytes, T lymphocytes, monocytes and granulocytes; it is also present on a subset of NK cells and certain hematopoietic malignant cells.
Purification notesThe purified antibody (protein A (G) affinity chromatography before conjugation) is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: Use 20µl reagent / 100µl of whole blood or 106 cells in a suspension. (sufficient for 100 tests).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionCell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.
Tissue specificityExpressed in B cell lines and T lymphocytes.
Sequence similaritiesBelongs to the selectin/LECAM family. Contains 1 C-type lectin domain. Contains 1 EGF-like domain. Contains 2 Sushi (CCP/SCR) domains.
Flow Cytometry analysis of Human peripheral blood cells labeling CD62L with Anti-CD62L antibody [LT-TD180] (Phycoerythrin) (ab77117).
References for Anti-CD62L antibody [LT-TD180] (Phycoerythrin) (ab77117)
This product has been referenced in:
Linnebacher M et al. Identification of an MSI-H tumor-specific cytotoxic T cell epitope generated by the (-1) frame of U79260(FTO). J Biomed Biotechnol2010:841451 (2010).
Read more (PubMed: 20339516) »