Anti-CD62L antibody [MEL-14] (Allophycocyanin/Cy7 ®) (ab81993)

Overview

  • Product name
    Anti-CD62L antibody [MEL-14] (Allophycocyanin/Cy7 ®)
    See all CD62L primary antibodies
  • Description
    Rat monoclonal [MEL-14] to CD62L (Allophycocyanin/Cy7 ®)
  • Conjugation
    Allophycocyanin/Cy7 ®. Ex: 650nm, Em: 774nm
  • Tested applications
    Suitable for: Flow Cyt, IP, IHC-Fr, Functional Studiesmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    C3H/eb cloned Mouse B lymphoma 38C-13

  • General notes

    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

    Clone MEL-14 blocks in vitro binding of lymphocytes to peripheral lymph node HEV and inhibits in vivo lymphocyte extravasation into peripheral lymph nodes and the late stages of leukocyte rolling.

Properties

Applications

Our Abpromise guarantee covers the use of ab81993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
IP Use at an assay dependent concentration. PubMed: 6866086
IHC-Fr Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration. PubMed: 6866086

Target

  • Function
    Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.
  • Tissue specificity
    Expressed in B cell lines and T lymphocytes.
  • Sequence similarities
    Belongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 2 Sushi (CCP/SCR) domains.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • A.11 antibody
    • AI528707 antibody
    • CD62 antigen ligand antibody
    • CD62 antigen-like family member L antibody
    • CD62L antibody
    • gp90-MEL antibody
    • IgA nephropathy, susceptibility to, included antibody
    • L Selectin antibody
    • L-selectin antibody
    • LAM-1 antibody
    • LAM1 antibody
    • LECAM1 antibody
    • LEU8 antibody
    • Leukocyte adhesion molecule 1 antibody
    • Leukocyte surface antigen Leu-8 antibody
    • Leukocyte-endothelial cell adhesion molecule 1 antibody
    • Lnhr antibody
    • LSEL antibody
    • Ly-22 antibody
    • Ly-m22 antibody
    • Lyam-1 antibody
    • LYAM1 antibody
    • LYAM1_HUMAN antibody
    • Lymph node homing receptor antibody
    • Lymphocyte adhesion molecule 1 antibody
    • Lymphocyte antigen 22 antibody
    • Lymphocyte surface MEL-14 antigen antibody
    • MEL-14 antibody
    • Pln homing receptor antibody
    • PLNHR antibody
    • Selectin L antibody
    • Selectin, lymphocyte antibody
    • SELL antibody
    • TQ1 antibody
    see all

Images

  • Flow Cytometry of C57Bl/6 splenocytes labeling CD62L useing ab81993 at 0.06µg (solid line) or Rat IgG2a isotype control at 0.06µg (dashed line).

References

This product has been referenced in:
  • Iwabuchi K  et al. Distribution of MEL-14+ cells in various lymphoid tissues. Immunobiology 182:161-73 (1991). Flow Cyt, Functional Studies ; Mouse . Read more (PubMed: 1909300) »
  • Siegelman MH  et al. The mouse lymph node homing receptor is identical with the lymphocyte cell surface marker Ly-22: role of the EGF domain in endothelial binding. Cell 61:611-22 (1990). Flow Cyt, Functional Studies, IP ; Mouse . Read more (PubMed: 1693096) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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