The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
FunctionCell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.
Tissue specificityExpressed in B cell lines and T lymphocytes.
Sequence similaritiesBelongs to the selectin/LECAM family. Contains 1 C-type lectin domain. Contains 1 EGF-like domain. Contains 2 Sushi (CCP/SCR) domains.
Flow cytometry of C57Bl/6 splenocytes labeling CD62L with ab93565 at 0.25µg (solid line), or Rat IgG2a isotype control at 0.25µg (dashed line).
References for Anti-CD62L antibody [MEL-14] (PerCP/Cy5.5®) (ab93565)
This product has been referenced in:
Siegelman MH et al. The mouse lymph node homing receptor is identical with the lymphocyte cell surface marker Ly-22: role of the EGF domain in endothelial binding. Cell61:611-22 (1990).
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