The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: Use 0.06 - 0.125 µg for 105 to 108 cells in a final volume of 100 µl.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionCell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.
Tissue specificityExpressed in B cell lines and T lymphocytes.
Sequence similaritiesBelongs to the selectin/LECAM family. Contains 1 C-type lectin domain. Contains 1 EGF-like domain. Contains 2 Sushi (CCP/SCR) domains.
Staining of BALB/c splenocytes with 0.125 µg of PerCP-Cy5.5 Rat IgG2a Isotype Control (open histogram) or 0.06 µg of ab93565 (colored histogram). Total viable cells were used for analysis.
References for Anti-CD62L antibody [MEL-14] (PerCP/Cy5.5®) (ab93565)
This product has been referenced in:
Siegelman MH et al. The mouse lymph node homing receptor is identical with the lymphocyte cell surface marker Ly-22: role of the EGF domain in endothelial binding. Cell61:611-22 (1990).
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