Anti-CD62L antibody [OX-85] (Phycoerythrin) (ab33924)

Overview

  • Product name
    Anti-CD62L antibody [OX-85] (Phycoerythrin)
    See all CD62L primary antibodies
  • Description
    Mouse monoclonal [OX-85] to CD62L (Phycoerythrin)
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notes


    In rats, CD62L is expressed by a subset of thymocytes, predominantly single positive CD4 or CD8 cells. Expression of rat CD62L is weak, and sensitive detection systems (for example, three layer biotin-streptavidin techniques) may be required.

Properties

Applications

Our Abpromise guarantee covers the use of ab33924 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/1 - 1/10. Use 10µl of the suggested working dilution to label 106 cells.



ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Cell surface adhesion protein. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.
  • Tissue specificity
    Expressed in B cell lines and T lymphocytes.
  • Sequence similarities
    Belongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 2 Sushi (CCP/SCR) domains.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • A.11 antibody
    • AI528707 antibody
    • CD62 antigen ligand antibody
    • CD62 antigen-like family member L antibody
    • CD62L antibody
    • gp90-MEL antibody
    • IgA nephropathy, susceptibility to, included antibody
    • L Selectin antibody
    • L-selectin antibody
    • LAM-1 antibody
    • LAM1 antibody
    • LECAM1 antibody
    • LEU8 antibody
    • Leukocyte adhesion molecule 1 antibody
    • Leukocyte surface antigen Leu-8 antibody
    • Leukocyte-endothelial cell adhesion molecule 1 antibody
    • Lnhr antibody
    • LSEL antibody
    • Ly-22 antibody
    • Ly-m22 antibody
    • Lyam-1 antibody
    • LYAM1 antibody
    • LYAM1_HUMAN antibody
    • Lymph node homing receptor antibody
    • Lymphocyte adhesion molecule 1 antibody
    • Lymphocyte antigen 22 antibody
    • Lymphocyte surface MEL-14 antigen antibody
    • MEL-14 antibody
    • Pln homing receptor antibody
    • PLNHR antibody
    • Selectin L antibody
    • Selectin, lymphocyte antibody
    • SELL antibody
    • TQ1 antibody
    see all

Images

  • Overlay histogram showing mouse peritoneal cells stained with ab33924 (red line). Cells were gated on CD3+ cells. The cells were diluted 1/100 in PBS/BSA 1% and incubated for 1 hour at 4ºC. Isotype control antibody (black line) was used under the same conditions.

References

ab33924 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for submitting an Abreview for ab33924. As you may have noticed, your review has now been published on our website.
Since you obtained poor results using the antibody in an untested species (dog), we would like to follow up on this to see...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Dog Cell (from whole blood)
Specification
from whole blood
Preparation
Cell harvesting/tissue preparation method: after red blood cell lysis
Sample buffer: PBS/BSA 1%
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
alive lymphocytes
Username

Abcam user community

Verified customer

Submitted Nov 06 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Cynomolgus Monkey Cell (Whole blood)
Specification
Whole blood
Preparation
Cell harvesting/tissue preparation method: After red blood cell lysis
Sample buffer: PBS/BSA 1%/Azide 0.01%
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
alive lymphocytes
Username

Abcam user community

Verified customer

Submitted Apr 27 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Rat Cell (Splenocytes)
Specification
Splenocytes
Preparation
Cell harvesting/tissue preparation method: spleen was mashed
Sample buffer: PBS/BSA 1%/Azide 0.01%
Permeabilization
No
Gating Strategy
alive lymphocytes
Username

Abcam user community

Verified customer

Submitted Apr 19 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Mouse Cell (cell from peritoneal lavage (after thioglycolate))
Specification
cell from peritoneal lavage (after thioglycolate)
Preparation
Cell harvesting/tissue preparation method: PBS lavage of the peritoneal cavity
Sample buffer: PBS/BSA 1%
Permeabilization
No
Gating Strategy
CD3+ cells
Username

Abcam user community

Verified customer

Submitted Nov 28 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (PBMC)
Specification
PBMC
Preparation
Cell harvesting/tissue preparation method: Ficoll
Sample buffer: PBS/BSA 1%
Permeabilization
No
Gating Strategy
CD3+ cells
Username

Abcam user community

Verified customer

Submitted Nov 28 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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