Overview

  • Product nameCD62L (Selectin L) Human ELISA Kit
    See all CD62L kits
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 1 ng/ml
  • Range
    1.6 ng/ml - 60 ng/ml
  • Assay time
    1h 45m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s CD62L (Selectin L) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CD62L in Human serum, plasma, buffered solutions and cell culture medium.

    A monoclonal antibody specific for CD62L has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD62L concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for CD62L are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of CD62L present in the samples. 

    This kit will recognize both endogenous and recombinant Human CD62L.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceCD62L is a cell surface component that is a member of a family of adhesion/homing receptors. These play important roles in leukocyte-endothelial cell interactions. CD62L is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4 binding proteins.
  • Cellular localizationCell Membrane; single-pass type I membrane protein.
  • Alternative names
    • A.11
    • AI528707
    • CD62 antigen ligand
    • CD62 antigen-like family member L
    • CD62L antigen
    • gp90 MEL
    • hLHRc
    • IgA nephropathy, susceptibility to, included
    • L Selectin
    • L selectin precursor
    • LAM 1
    • LAM1
    • LECAM1
    • Leu 8
    • Leu8
    • Leukocyte adhesion molecule 1
    • Leukocyte endothelial cell adhesion molecule 1
    • Leukocyte surface antigen Leu 8
    • Leukocyte surface antigen Leu8
    • Leukocyte-endothelial cell adhesion molecule 1
    • LNHR
    • LSEL
    • Ly-22
    • Ly-m22
    • Lyam 1
    • LYAM1
    • Lymph node homing receptor
    • Lymphocyte adhesion molecule 1
    • Lymphocyte antigen 22
    • Lymphocyte surface MEL-14 antigen
    • PLNHR
    • Selectin L
    • Selectin L (lymphocyte adhesion molecule 1)
    • Selectin, lymphocyte
    • SELL
    • TQ1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab45917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

CD62L (Selectin L) Human ELISA Kit images

  • Representative Standard Curve using ab45917

Protocols

References for CD62L (Selectin L) Human ELISA Kit (ab45917)

This product has been referenced in:
  • Chen R  et al. Differentially expressed RNA from public microarray data identifies serum protein biomarkers for cross-organ transplant rejection and other conditions. PLoS Comput Biol 6: (2010). ELISA ; Human . Read more (PubMed: 20885780) »

See 1 Publication for this product

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