Overview

  • Product nameAnti-CD62P antibody
    See all CD62P primary antibodies
  • Description
    Rabbit polyclonal to CD62P
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 600 - 700 of Human CD62P.

  • Positive control
    • This antibody gave a positive signal in Human Platelet whole cell lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human tonsil.

Properties

Applications

Our Abpromise guarantee covers the use of ab119435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 140 kDa (predicted molecular weight: 90 kDa).

Target

  • FunctionCa(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1.
  • Tissue specificityStored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
  • Involvement in diseaseDefects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.
  • Sequence similaritiesBelongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 9 Sushi (CCP/SCR) domains.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Antigen CD62 antibody
    • CD62 antibody
    • CD62 antigen-like family member P antibody
    • CD62P antibody
    • GMP 140 antibody
    • GMP-140 antibody
    • GMP140 antibody
    • GMRP antibody
    • Granule membrane protein 140 antibody
    • Granulocyte membrane protein antibody
    • GRMP antibody
    • LECAM 3 antibody
    • LECAM3 antibody
    • Leukocyte endothelial cell adhesion molecule 3 antibody
    • Leukocyte-endothelial cell adhesion molecule 3 antibody
    • LYAM3_HUMAN antibody
    • P Selectin antibody
    • P-selectin antibody
    • PADGEM antibody
    • Platelet activation dependent granule-external membrane protein antibody
    • Platelet alpha granule membrane protein antibody
    • PSEL antibody
    • Selectin P (granule membrane protein 140kDa, antigen CD62) antibody
    • SELP antibody
    see all

Anti-CD62P antibody images

  • Anti-CD62P antibody (ab119435) at 1 µg/ml + Platelet (Human) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 90 kDa
    Observed band size : 140 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 49 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 90 seconds

    The CD62P protein has a predicted molecular weight of 90 kDa, however it is extensively glycosylated and has been shown to run in the 140 kDa region (SwissProt data).

    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab119435 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

  • IHC image of CD62P staining in Human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab119435, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-CD62P antibody (ab119435)

ab119435 has not yet been referenced specifically in any publications.

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