• Product name
  • Description
    Rabbit polyclonal to CD63
  • Host species
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human CD63.

    (Peptide available as ab93074.)

  • Positive control
    • This antibody gave a positive signal in Human platelet and HepG2 whole cell lysates.



Our Abpromise guarantee covers the use of ab68418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 24,43 kDa (predicted molecular weight: 25 kDa).


  • Function
    This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation.
  • Tissue specificity
    Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.
  • Sequence similarities
    Belongs to the tetraspanin (TM4SF) family.
  • Cellular localization
    Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • Lysosomal associated membrane protein 3 antibody
    • CD 63 antibody
    • CD63 antibody
    • CD63 antigen (melanoma 1 antigen) antibody
    • CD63 antigen antibody
    • CD63 antigen melanoma 1 antigen antibody
    • CD63 molecule antibody
    • CD63_HUMAN antibody
    • gp55 antibody
    • Granulophysin antibody
    • LAMP 3 antibody
    • LAMP-3 antibody
    • LAMP3 antibody
    • LIMP antibody
    • Lysosomal-associated membrane protein 3 antibody
    • Lysosome associated membrane glycoprotein 3 antibody
    • Mast cell antigen AD1 antibody
    • ME491 antibody
    • Melanoma 1 antigen antibody
    • Melanoma associated antigen ME491 antibody
    • Melanoma associated antigen MLA1 antibody
    • Melanoma-associated antigen ME491 antibody
    • MGC72893 antibody
    • MLA 1 antibody
    • MLA1 antibody
    • NGA antibody
    • Ocular melanoma associated antigen antibody
    • Ocular melanoma-associated antigen antibody
    • OMA81H antibody
    • PTLGP40 antibody
    • Tetraspanin 30 antibody
    • Tetraspanin-30 antibody
    • Tspan 30 antibody
    • Tspan-30 antibody
    • TSPAN30 antibody
    see all


  • Anti-CD63 antibody (ab68418) at 1 µg/ml + Platelet (Human) Whole Cell Lysate at 10 µg

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 25 kDa
    Observed band size: 43 kDa (why is the actual band size different from the predicted?)

    Exposure time: 8 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab68418 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

    CD63 has a predicted molecular weight of 25 kDa; however it has a number of potential glycosylation sites which may affect the migration of the protein (SwissProt data). This is consistent with the literature which describes a band between 30-60 kDa in human platelets(PMID:1993697).

  • Anti-CD63 antibody (ab68418) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 25 kDa
    Observed band size: 24 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 145 kDa, 42 kDa (possible glycosylated form). We are unsure as to the identity of these extra bands.

    Exposure time: 20 minutes

    The 42 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to human CD63 antigen.


This product has been referenced in:
  • Le M  et al. Exosomes are released by bystander cells exposed to radiation-induced biophoton signals: Reconciling the mechanisms mediating the bystander effect. PLoS One 12:e0173685 (2017). Read more (PubMed: 28278290) »
  • Wang L  et al. Exosomes derived from pancreatic cancer cells induce insulin resistance in C2C12 myotube cells through the PI3K/Akt/FoxO1 pathway. Sci Rep 7:5384 (2017). Read more (PubMed: 28710412) »

See all 6 Publications for this product

Customer reviews and Q&As

The one CD63 antibody currently in our catalogue that has been shown to be reactive with rat is ab108950, the mouse clone AD1.


It is also available as a FITC conjugate, ab108949. Read More

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Serum (Purified microvesicles)
Loading amount
1 µg
Purified microvesicles
Gel Running Conditions
Reduced Denaturing (12.5% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 12 2012

Thank you for your inquiry. Unfortunately, we do not have ab68418 conjugated to biotin. However, I would like to recommend some conjugation kits that we have in our catalog : www.abcam.com/easylink I hope this information is nevertheless helpful ...

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