Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab68418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 24,43 kDa (predicted molecular weight: 25 kDa).

Target

  • FunctionThis antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation.
  • Tissue specificityDysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.
  • Sequence similaritiesBelongs to the tetraspanin (TM4SF) family.
  • Cellular localizationCell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • Lysosomal associated membrane protein 3 antibody
    • CD 63 antibody
    • CD63 antibody
    • CD63 antigen (melanoma 1 antigen) antibody
    • CD63 antigen antibody
    • CD63 antigen melanoma 1 antigen antibody
    • CD63 molecule antibody
    • CD63_HUMAN antibody
    • gp55 antibody
    • Granulophysin antibody
    • LAMP 3 antibody
    • LAMP-3 antibody
    • LAMP3 antibody
    • LIMP antibody
    • Lysosomal-associated membrane protein 3 antibody
    • Lysosome associated membrane glycoprotein 3 antibody
    • Mast cell antigen AD1 antibody
    • ME491 antibody
    • Melanoma 1 antigen antibody
    • Melanoma associated antigen ME491 antibody
    • Melanoma associated antigen MLA1 antibody
    • Melanoma-associated antigen ME491 antibody
    • MGC72893 antibody
    • MLA 1 antibody
    • MLA1 antibody
    • NGA antibody
    • Ocular melanoma associated antigen antibody
    • Ocular melanoma-associated antigen antibody
    • OMA81H antibody
    • PTLGP40 antibody
    • Tetraspanin 30 antibody
    • Tetraspanin-30 antibody
    • Tspan 30 antibody
    • Tspan-30 antibody
    • TSPAN30 antibody
    see all

Anti-CD63 antibody images

  • Anti-CD63 antibody (ab68418) at 1 µg/ml + Platelet (Human) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 25 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)


    Exposure time : 8 minutes

    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab68418 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

    CD63 has a predicted molecular weight of 25 kDa; however it has a number of potential glycosylation sites which may affect the migration of the protein (SwissProt data). This is consistent with the literature which describes a band between 30-60 kDa in human platelets(PMID:1993697).

  • Anti-CD63 antibody (ab68418) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 25 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 145 kDa,42 kDa (possible glycosylated form). We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

    The 42 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to human CD63 antigen.

References for Anti-CD63 antibody (ab68418)

This product has been referenced in:
  • Rosas LE  et al. In vitro immunotoxicity assessment of culture-derived extracellular vesicles in human monocytes. J Immunotoxicol 13:652-65 (2016). Read more (PubMed: 27075513) »

See 1 Publication for this product

Product Wall

The one CD63 antibody currently in our catalogue that has been shown to be reactive with rat is ab108950, the mouse clone AD1.

http://www.abcam.com/index.html?datasheet=108950

It is also available as a FITC conjugate, ab108949. Read More

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Serum (Purified microvesicles)
Loading amount 1 µg
Specification Purified microvesicles
Gel Running Conditions Reduced Denaturing (12.5% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 12 2012

Thank you for your inquiry. Unfortunately, we do not have ab68418 conjugated to biotin. However, I would like to recommend some conjugation kits that we have in our catalog : www.abcam.com/easylink I hope this information is nevertheless helpful ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"