Overview

  • Product name
    Anti-CD63 antibody [MEM-259]
    See all CD63 primary antibodies
  • Description
    Mouse monoclonal [MEM-259] to CD63
  • Specificity
    CD63 antigen (human)
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, ICC/IFmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation (Human). (HPB-ALL T cell line)

  • Positive control
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human skin melanoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab8219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 2 µg/ml.

Use at an assay dependant concentration. For detection of activated platelets, neutrophils and basophils.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P Use a concentration of 10 µg/ml.
ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation.
    • Tissue specificity
      Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.
    • Sequence similarities
      Belongs to the tetraspanin (TM4SF) family.
    • Cellular localization
      Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules.
    • Information by UniProt
    • Database links
    • Alternative names
      • Lysosomal associated membrane protein 3 antibody
      • CD 63 antibody
      • CD63 antibody
      • CD63 antigen (melanoma 1 antigen) antibody
      • CD63 antigen antibody
      • CD63 antigen melanoma 1 antigen antibody
      • CD63 molecule antibody
      • CD63_HUMAN antibody
      • gp55 antibody
      • Granulophysin antibody
      • LAMP 3 antibody
      • LAMP-3 antibody
      • LAMP3 antibody
      • LIMP antibody
      • Lysosomal-associated membrane protein 3 antibody
      • Lysosome associated membrane glycoprotein 3 antibody
      • Mast cell antigen AD1 antibody
      • ME491 antibody
      • Melanoma 1 antigen antibody
      • Melanoma associated antigen ME491 antibody
      • Melanoma associated antigen MLA1 antibody
      • Melanoma-associated antigen ME491 antibody
      • MGC72893 antibody
      • MLA 1 antibody
      • MLA1 antibody
      • NGA antibody
      • Ocular melanoma associated antigen antibody
      • Ocular melanoma-associated antigen antibody
      • OMA81H antibody
      • PTLGP40 antibody
      • Tetraspanin 30 antibody
      • Tetraspanin-30 antibody
      • Tspan 30 antibody
      • Tspan-30 antibody
      • TSPAN30 antibody
      see all

    Images

    • ab8219 staining CD63 in human fibrosarcoma cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde. Samples were incubated with primary antibody which was used diluted to 10 µg/ml for 1 hour at 20°C. An Alexa Fluor®555-conjugated donkey monoclonal to mouse IgG was used at dilution at 1/500 as secondary antibody.

      See Abreview

    • ab8219 staining CD63 in human Fibrosarcoma HT1080 cell line by flow cytometry. Cells were incubated with primary antibody for 1 hour. ab7003, a donkey polyclonal to mouse Ig, was used as the secondary antibody.

      See Abreview

    • IHC image of CD63 staining in Human skin melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8219, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Flow cytometry analysis staining CD63 in IgE- activated peripheral blood using ab8219 (2μg/ml). 

    References

    This product has been referenced in:
    • Yang Y  et al. Secretory carrier membrane protein 5 is an autophagy inhibitor that promotes the secretion of a-synuclein via exosome. PLoS One 12:e0180892 (2017). WB ; Rat, Human . Read more (PubMed: 28700687) »
    • Su Y  et al. PDLIM5 links kidney anion exchanger 1 (kAE1) to ILK and is required for membrane targeting of kAE1. Sci Rep 7:39701 (2017). Read more (PubMed: 28045035) »

    See all 33 Publications for this product

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample
    Human Cell (Blood film)
    Specification
    Blood film
    Permeabilization
    Yes - 0.1M PBS with 1% TritonX100
    Fixative
    Paraformaldehyde
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Sep 26 2014

    ab2215 should be used at a concentration of 2-4 ug/ml in blocking buffer when incubating your membrane whereas ab8216 should be used at a concentration of 2 ug/ml.

    The gel should be run in non-reducing conditions as these antibodies do not re...

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    We have not tested the stability of ab8219 under the alkaline conditions that you described; thus, we cannot guarantee that it will work under these conditions. The laboratory thinks it is possible that it could work, but only performing the exper...

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    We are not aware of a reference describing production and characterization of this CD63 antibody. The epitope the antibody recognizes on CD63 has not, to our knowledge, been mapped, but we do know that the epitope is extracellular when CD63 is integrat...

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    Thank you for contacting us.

    The antibodies have not been tested for reactivity with bovine samples, and we have not received reports of bovine reactivity from our customers.

    Please do not hesitate to contact us if you need any mo...

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    Thank you for your inquiry.

    Ab8219 (anti-CD63) is supplied as 100ul at a concentration of 1mg/ml. We have a customer who reported successfully using the antibody at a 1/200 dilution (0.2 mg/ml), but it may be necessary to optimize this for you...

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    Thank you for your enquiry.

    I can confirm that the epitope for ab8219 CD63 antibody [MEM-259] has been mapped to the extracellular region of the CD63 protein.

    I hope this will be helpful to you. If you have any further questions, ...

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    Thnak you for contacting Abcam.

    In regards to ab8219, I have had the chance to talk with the lab. According to them this antigen is indeed very variable on WB of various material. I am sending the pattern they obtained on non-reducing K562 c...

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    Thank you for contacting us.

    CD63 antigen is also known as LIMP and LAMP-3. While unmodified protein is about 25kDA, this is heavily glycolylated. AB59476migrates like a smear due to a very complexe N-glycosylation. I would recommend this ...

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    The antibody was validated for western blotting using these conditions:

    Sample: K562 cells (24 millions, lysed with 1% laurylmaltoside in 700 ul), non-reducing SDS, denatured by heat for 5 min.
    Blocking: 5% milk, 4oC overnight
    Prim...

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