Overview

  • Product name
    Anti-CD63 antibody [MEM-259] (PerCP)
    See all CD63 primary antibodies
  • Description
    Mouse monoclonal [MEM-259] to CD63 (PerCP)
  • Conjugation
    PerCP. Ex: 482nm, Em: 675nm
  • Specificity
    ab77227 reacts with CD63 expressed by granulocytes, platelets, T cells, monocytes/macrophages and endothelial cells.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    HPB-ALL T cell line

  • General notes

    The antibody is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions.

Properties

Applications

Our Abpromise guarantee covers the use of ab77227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: The reagent is designed for Flow Cytometry analysis of human blood cells
    Use 10 µl/ 100 µl of whole blood or 106 cells in suspension.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation.
    • Tissue specificity
      Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.
    • Sequence similarities
      Belongs to the tetraspanin (TM4SF) family.
    • Cellular localization
      Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules.
    • Information by UniProt
    • Database links
    • Alternative names
      • Lysosomal associated membrane protein 3 antibody
      • CD 63 antibody
      • CD63 antibody
      • CD63 antigen (melanoma 1 antigen) antibody
      • CD63 antigen antibody
      • CD63 antigen melanoma 1 antigen antibody
      • CD63 molecule antibody
      • CD63_HUMAN antibody
      • gp55 antibody
      • Granulophysin antibody
      • LAMP 3 antibody
      • LAMP-3 antibody
      • LAMP3 antibody
      • LIMP antibody
      • Lysosomal-associated membrane protein 3 antibody
      • Lysosome associated membrane glycoprotein 3 antibody
      • Mast cell antigen AD1 antibody
      • ME491 antibody
      • Melanoma 1 antigen antibody
      • Melanoma associated antigen ME491 antibody
      • Melanoma associated antigen MLA1 antibody
      • Melanoma-associated antigen ME491 antibody
      • MGC72893 antibody
      • MLA 1 antibody
      • MLA1 antibody
      • NGA antibody
      • Ocular melanoma associated antigen antibody
      • Ocular melanoma-associated antigen antibody
      • OMA81H antibody
      • PTLGP40 antibody
      • Tetraspanin 30 antibody
      • Tetraspanin-30 antibody
      • Tspan 30 antibody
      • Tspan-30 antibody
      • TSPAN30 antibody
      see all

    References for Anti-CD63 antibody [MEM-259] (PerCP) (ab77227)

    This product has been referenced in:
    • Qin Y  et al. Bone marrow stromal/stem cell-derived extracellular vesicles regulate osteoblast activity and differentiation in vitro and promote bone regeneration in vivo. Sci Rep 6:21961 (2016). Read more (PubMed: 26911789) »
    • Cerny J  et al. The small chemical vacuolin-1 inhibits Ca(2+)-dependent lysosomal exocytosis but not cell resealing. EMBO Rep 5:883-8 (2004). Read more (PubMed: 15332114) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Flow Cytometry
    Fixation
    no fixation
    Permeabilization
    No
    Sample
    Human Cell (basophile granulocytes)
    Specification
    basophile granulocytes
    Gating Strategy
    leucocyte gate
    Preparation
    Cell harvesting/tissue preparation method: peripheral blood cells, separated by Ficoll density separation, followed by magnetic isolation
    Sample buffer: PBS with human IgG (Fc-block)
    Username

    Abcam user community

    Verified customer

    Submitted Aug 15 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    Human Cell (human blood)
    Specification
    human blood
    Fixation
    none
    Permeabilization
    No
    Gating Strategy
    granulocytes, lymphocytes, monocytes
    Username

    Abcam user community

    Verified customer

    Submitted Jun 07 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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