The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 10µl for 106 cells. ab37391-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
The high affinity receptor for immunoglobulin G CD64 is encoded by a family of 3 genes that share over 98% of DNA sequence homology: FCGR1A, FCGR1B and FCGR1C. The FCGR1C gene, like FCGR1A, is located on 1q21. The third gene of this family, FCGR1B, was found at 1p12. CD64 plays a pivotal role in the immune response. It is a glycoprotein (MW 72 kD) that is constitutively expressed on human monocytes and macrophages.
Cell membrane; Single-pass type I membrane protein.