The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/20 - 1/200.
Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab91545-Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration.
RelevanceCD65s is aberrantly expressed on some acute myeloid leukaemias (AML). During normal myelopoiesis, expression of CD65s follows the disappearance of the progenitor antigen CD34. CD65s is the sialylated form of human CD65 and is a leucocyte carbohydrate antigen expressed by granulocytes, monocytes and leukaemic cells of myelomonocytic lineage.
Anti-CD65s antibody [VIM-2] images
Flow Cytometry - CD65s antibody [VIM-2] (ab74081)
Staining of mouse peripheral blood granulocytes with ab74081 at a 1/20 dilution.
References for Anti-CD65s antibody [VIM-2] (ab74081)
has not yet been referenced specifically in any publications.
Publishing research using ab74081? Please let us know so that we can cite the reference in this datasheet.
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