Overview

  • Product name
    Anti-CD65s antibody [VIM-2] (FITC)
  • Description
    Mouse monoclonal [VIM-2] to CD65s (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    THP1 (human acute monocytic leukaemia cells).

  • General notes


    Clone VIM-2 has been reliably used as a marker for distinguishing between mature and undifferentiated AML. Cross-linking of the CD65s antigen using clone VIM-2, has been shown to induce phagocyte cytoplasmic calcium flux, oxidative burst and degranulation

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Primary antibody notes
    Clone VIM-2 has been reliably used as a marker for distinguishing between mature and undifferentiated AML. Cross-linking of the CD65s antigen using clone VIM-2, has been shown to induce phagocyte cytoplasmic calcium flux, oxidative burst and degranulation
  • Clonality
    Monoclonal
  • Clone number
    VIM-2
  • Myeloma
    NS1
  • Isotype
    IgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab74080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/1 - 1/10.

Use 10µl of the suggested working dilution to label 106 cells in 100µl.





ab91546-Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

Target

  • Relevance
    CD65s is aberrantly expressed on some acute myeloid leukaemias (AML). During normal myelopoiesis, expression of CD65s follows the disappearance of the progenitor antigen CD34. CD65s is the sialylated form of human CD65 and is a leucocyte carbohydrate antigen expressed by granulocytes, monocytes and leukaemic cells of myelomonocytic lineage.
  • Alternative names
    • CDw65 antibody

Anti-CD65s antibody [VIM-2] (FITC) images

  • Staining of human peripheral blood granulocytes with ab74080 at 1/10 dilution.

References for Anti-CD65s antibody [VIM-2] (FITC) (ab74080)

This product has been referenced in:
  • Kniep B  et al. The CDw65 monoclonal antibodies VIM-8 and VIM-11 bind to the neutral glycolipid V3FucnLc8Cer. J Biochem 119:456-62 (1996). Read more (PubMed: 8830039) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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