SpecificityThe monoclonal antibody is directed against the CD66acde antigen. The monoclonal antibody reacts with 100% of mature human peripheral granulocytes. It reacts weak with malignant cells of patients with B cell derived Chronic Lymphoid Leukaemia (CLL). The monoclonal antibody does not react with normal human peripheral B cells, T cells, monocytes and platelets. In immunohistology the monoclonal antibody reacts with some tissue macrophages and CEA, the Carcino Embryonic Antigen, expressed on
colon carcinoma and other carcinomas.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesFlow Cyt: Use at an assay dependent dilution.
Study of mature granulocytes in peripheral blood.
Indirect immunofluorescence staining with analysis by flow cytometry or
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionCell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins.
Tissue specificityFound in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.
Sequence similaritiesBelongs to the immunoglobulin superfamily. CEA family. Contains 7 Ig-like (immunoglobulin-like) domains.
Post-translational modificationsComplex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate.