Overview

  • Product nameAnti-CD68 antibody [EPR1392Y]
    See all CD68 primary antibodies
  • Description
    Rabbit monoclonal [EPR1392Y] to CD68
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human CD68 aa 1-100 (N terminal).

  • Positive control
    • SH-SY-5Y cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Alternative versions available:

    Anti-CD68 antibody (Alexa Fluor® 488) [EPR1392Y] (ab197519)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone numberEPR1392Y
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).

For unpurified, use 1/500 - 1/1000.

  • Application notesIs unsuitable for ICC/IF or IHC-P.
  • Target

    • FunctionCould play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
    • Tissue specificityHighly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
    • Sequence similaritiesBelongs to the LAMP family.
    • Post-translational
      modifications
      N- and O-glycosylated.
    • Cellular localizationCell membrane and Endosome membrane. Lysosome membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 68 antibody
      • CD68 antibody
      • CD68 antigen antibody
      • CD68 molecule antibody
      • CD68_HUMAN antibody
      • DKFZp686M18236 antibody
      • gp11 antibody
      • Gp110 antibody
      • LAMP4 antibody
      • Macrophage antigen CD68 (microsialin) antibody
      • MACROPHAGE ANTIGEN CD68 antibody
      • Macrosialin antibody
      • SCARD1 antibody
      • Scavenger receptor class D member 1 antibody
      see all

    Anti-CD68 antibody [EPR1392Y] images

    • All lanes : Anti-CD68 antibody [EPR1392Y] (ab76308) at 1/2000 dilution (purified)

      Lane 1 : L292 cell lysate
      Lane 2 : Neuro-2a cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 37 kDa
      Observed band size : 37 kDa

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Anti-CD68 antibody [EPR1392Y] (ab76308) at 1/2000 dilution (purified) + PC-12 cell lysate at 20 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 37 kDa
      Observed band size : 37 kDa

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Anti-CD68 antibody [EPR1392Y] (ab76308) at 1/10000 dilution (purified) + Jurkat cell lysate at 20 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 37 kDa
      Observed band size : 37 kDa

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Anti-CD68 antibody [EPR1392Y] (ab76308) at 1/2000 dilution (purified) + THP-1 cell lysate at 20 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size : 37 kDa
      Observed band size : 37 kDa

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Anti-CD68 antibody [EPR1392Y] (ab76308) at 1/500 dilution (unpurified) + SH-SY-5Y cell lysate at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/1000 dilution

      Predicted band size : 37 kDa
      Observed band size : 37 kDa

    References for Anti-CD68 antibody [EPR1392Y] (ab76308)

    This product has been referenced in:
    • Miyata T  et al. The roles of platelet-derived growth factors and their receptors in brain radiation necrosis. Radiat Oncol 9:51 (2014). IHC-P ; Human . Read more (PubMed: 24512807) »
    • Yu L  et al. Genetic and pharmacological inhibition of galectin-3 prevents cardiac remodeling by interfering with myocardial fibrogenesis. Circ Heart Fail 6:107-17 (2013). WB ; Rat . Read more (PubMed: 23230309) »

    See all 4 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample Rat Tissue sections (Liver)
    Specification Liver
    Blocking step (agent) for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Fixative Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Oct 15 2013

    Application Immunohistochemistry (Frozen sections)
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Sample Mouse Tissue sections (Lung)
    Specification Lung
    Permeabilization No
    Fixative Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Oct 10 2013

    Thank you for confirming those details. I am sorry to hear that the protocol tips I provided did not result in an improvement of the results.

    As requested I have arranged for a vial of ab31630 to be added to one of your pending orders: xxxxx,...

    Read More

    Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

    The details provided will enable us to investigate this case and will provide us with vital information for...

    Read More

    Thank you for your reply.

    The information regarding western blot on the datasheet for ab125212 is provided as the detection limit in western blot for this particular antibody is known to be 0.1ng/lane. For other antibodies, the detection lim...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"