Overview

  • Product name
    Anti-CD68 antibody [FA-11]
    See all CD68 primary antibodies
  • Description
    Rat monoclonal [FA-11] to CD68
  • Specificity
    ab53444 detects surface CD68 at low levels in resident mouse peritoneal macrophages which can be enhanced with thioglycollate stimulation.
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WB, IP, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue, cells or virus corresponding to Mouse CD68.

  • Positive control
    • IHC-Fr: Mouse lung tissue sections. ICC/IF: RAW246.7 cell line.
  • General notes

    This antibody clone is manufactured by Abcam.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.40
    Preservative: 0.09% Sodium azide
    Constituent: PBS

    Please note that some batches of ab53444 may contain 0.4M arginine. Please contact Scientific Support for further information.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
  • Clonality
    Monoclonal
  • Clone number
    FA-11
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab53444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration. PubMed: 18971331
WB Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.
IP Use at an assay dependent concentration.
IHC-Fr Use a concentration of 0.1 - 5 µg/ml.
Flow Cyt 1/50 - 1/100.

Use 10µl of the suggested working dilution to label 106 cells in 100µl. Membrane permeabilisation is required for this application.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
  • Tissue specificity
    Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
  • Sequence similarities
    Belongs to the LAMP family.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localization
    Cell membrane and Endosome membrane. Lysosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 68 antibody
    • CD68 antibody
    • CD68 antigen antibody
    • CD68 molecule antibody
    • CD68_HUMAN antibody
    • DKFZp686M18236 antibody
    • gp11 antibody
    • Gp110 antibody
    • LAMP4 antibody
    • Macrophage antigen CD68 (microsialin) antibody
    • MACROPHAGE ANTIGEN CD68 antibody
    • Macrosialin antibody
    • SCARD1 antibody
    • Scavenger receptor class D member 1 antibody
    see all

Anti-CD68 antibody [FA-11] images

  • IHC image of CD68 staining in mouse lung frozen tissue section. The section was incubated with ab53444, 0.1µg/ml, overnight at 4C. A goat anti-mouse biotinylated secondary antibody was used to detect the primary, and visualized using an ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • ab53444 stained RAW246.7 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53444 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed (ab150165) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • ab53444 staining mouse heart tissue by Immunohistochemistry (IHC-frozen sections). Tissue underwent fixation in paraformaldehyde, no permeabilization and blocked in 1% serum for 10 minutes at 20°C. The primary antibody used undiluted and incubated with sample for 16 hours at 20°C. A Biotin conjugated goat polyclonal to rat Ig, diluted 1/100 was used as the secondary.  

    See Abreview

  • ab53444 at 1/100 dilution staining CD68 in mouse spleen tissue by immunohistochemistry (frozen sections). Sections were acetone fixed prior to blocking in 8% milk for 40 minutes at 36°C and then incubated with ab53444 for 20 hours at 4°C. A biotin conjugated goat polyclonal to rat Ig, diluted 1/400, was used as the secondary antibody.

    See Abreview

  • ab53444 staining CD68 in mouse spleen tissue by Immunohistochemistry (Frozen sections). Antibody was detected with HRP-conjugated Goat anti-Rat IgG, showing staining in the red pulp.
  • ab53444 staining CD68 in murine heart tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in acetone, permeabilized using 0.3% Triton, blocked with 10% serum for 30 minutes at 20°C, then incubated with ab53444 at a 1/1000 dilution for 16 hours at 4°C. The secondary used was a FITC conjugated goat anti-rat polyclonal, used at a 1/1000 dilution.

    See Abreview

  • Staining of permeabilised mouse peritoneal macrophages with Rat anti mouse CD68 (ab53444).

References for Anti-CD68 antibody [FA-11] (ab53444)

This product has been referenced in:
  • McGinnis GJ  et al. Neuroinflammatory and cognitive consequences of combined radiation and immunotherapy in a novel preclinical model. Oncotarget 8:9155-9173 (2017). ICC/IF ; Mouse . Read more (PubMed: 27893434) »
  • Panayiotou E  et al. C1q ablation exacerbates amyloid deposition: A study in a transgenic mouse model of ATTRV30M amyloid neuropathy. PLoS One 12:e0175767 (2017). IHC-P ; Mouse . Read more (PubMed: 28407005) »

See all 52 Publications for this product

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6.0
Permeabilization
Yes - TritonX-100
Specification
lung
Blocking step
Invitrogen DAB kit as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Formaldehyde
Username

Miss. Yuna Son

Verified customer

Submitted Mar 10 2016

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Sample
Rat Tissue sections (Spinal Cord section)
Specification
Spinal Cord section
Permeabilization
Yes - 0.2% Triton X-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 25 2015

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (Raw264.7)
Specification
Raw264.7
Treatment
100 ng/ml LPS
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Aug 19 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated
Sample
Mouse Tissue sections (Aorta)
Specification
Aorta
Permeabilization
No
Fixative
10% Formalin
Username

Abcam user community

Verified customer

Submitted Jul 25 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Sample
Mouse Tissue sections (Lung)
Specification
Lung
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Oct 04 2013

Thank you for contacting Abcam.

As we discussed on the phone, the immunogen sequences for ab53444, ab9089, and ab3283 are unknown due to all 3 immunogens arising from tissue and cell preparations.

For CD47 (ab9089 and ab3283) the huma...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lung)
Specification
Lung
Fixative
10% Neutral Buffered Formalin
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH6 (Ventana mRcc)
Permeabilization
No
Username

Abcam user community

Verified customer

Submitted Oct 18 2012

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab125212.

To check the status of the order please contact ...

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Thank you for contacting us.

Your credit note ID is XXXXXX.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used ...

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I will issue you replacement or credit note if you wish, however, i would just like to make some comments:

I was asking about the Rat AP-polymer kit because we usually like to check the compatibility with our products as a few cause trou...

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1-10 of 23 Abreviews or Q&A

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