Overview

  • Product name
    Anti-CD68 antibody [KP1]
    See all CD68 primary antibodies
  • Description
    Mouse monoclonal [KP1] to CD68
  • Tested applications
    Suitable for: ICC/IF, IHC-FoFr, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human
  • Positive control
    • Tonsil
  • General notes

    This product was changed from ascites to tissue culture supernatant on [14/07/17]. The following lots are from ascites and are still in stock as of [14/07/17] – [GR320733, GR298098]. Lot numbers higher than [GR320733] will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.099% Sodium azide
    Constituents: 0.9% Proprietary component, 99% Water
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    KP1
  • Myeloma
    unknown
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab955 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 18804859
IHC-FoFr Use at an assay dependent concentration.
IHC-P 1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Recommended heat mediated antigen retrieval in pressure cooker for 2-5 minutes or steaming of tissue sections for 45-60 minutes followed by cooling for 10 minutes and washing in distilled water.

In case of enzymatic epitope retrieval - digest with Pepsin enzyme for 30-60 seconds at RT.

IHC-Fr Use at an assay dependent concentration.

Target

  • Function
    Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
  • Tissue specificity
    Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
  • Sequence similarities
    Belongs to the LAMP family.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localization
    Cell membrane and Endosome membrane. Lysosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 68 antibody
    • CD68 antibody
    • CD68 antigen antibody
    • CD68 molecule antibody
    • CD68_HUMAN antibody
    • DKFZp686M18236 antibody
    • gp11 antibody
    • Gp110 antibody
    • LAMP4 antibody
    • Macrophage antigen CD68 (microsialin) antibody
    • MACROPHAGE ANTIGEN CD68 antibody
    • Macrosialin antibody
    • SCARD1 antibody
    • Scavenger receptor class D member 1 antibody
    see all

Images

  • ab955 staining CD68 in Human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 10 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in antibody diution reagent) for 30 minutes at 20°C. An undiluted HRP-conjugated Rat anti-mouse/rabbit polymer was used as the secondary antibody.

    See Abreview

  • ab955 staining CD68 in Human ulcerated Oral (Mucosa/Bone) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton-X 100 in PBS and blocked with 2.5% serum for 90 minutes at 25°C; antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were incubated with primary antibody (1/500 in 1% serum in PBS +0.01% Triton-X 100) for 16 hours at 4°C. A commercial IHC kit and DAB was used to visualize the staining.

    See Abreview

  • ab955 staining CD68 in Human lung cancer tissues by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formalin and blocked with 2% horse serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/100) for 1 hour at 21°C. An Alexa Fluor® 488-conjugated Donkey anti-mouse IgG polyclonal (1/500) was used as the secondary antibody. DAPI containing mounting medium was used for nuclear staining (blue) and anti-TREM-1 (1/500) used for TREM-1 staining (red)

  • Immunohistochemical analysis of tonsil labelling CD68 with ab955.

  • ab955 at a 1/2000 dilution staining CD68 from mouse lung tissue sections (new born pups) by immunohistochemistry (frozen sections). The antibody was incubated with the tissue for 24 hours and then detected using an Alexa-Fluor® 488 Goat anti-mouse IgG. The negative control was secondary antibody alone.

    See Abreview

  • ab955 staining CD68 in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% horse serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in a Tris buffer. Samples were incubated with primary antibody (1/100 in blocking buffer) for 15 hours at 4°C. An undiluted HRP-conjugated Horse polyclonal was used as the secondary antibody.

    See Abreview

  • ab955 staining CD68 in Rabbit iliac tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500 in Dako antibody diluent) for 4 hours at 25°C. An undiluted Beta-galactosidase-conjugated rabbit polyclonal was used as the secondary antibody.

    See Abreview

  • Formalin-fixed, paraffin embedded sections of mouse spleen were stained for CD68 with ab at 1/200 dilution in immunohistochemical analysis. Alexa Fluor 647® conjugated Donkey Anti-Mouse was used as secondary antibody at 1/200 dilution.

  • Immunohistochemical analysis of tonsil labelling CD68 with ab955.

  • ab955 staining CD68 in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde. Samples were then blocked with 10% serum for 3 hours at 22°C followed by incubation with the primary antibody at a 1/100 dilution for 16 hours at 4°C. An Alexa-Fluor® 568 conjugated goat anti-mouse polyclonal was used as secondary antibody at a 1/400 dilution.

    See Abreview

References

This product has been referenced in:
  • Kim J  et al. The Angiogenesis Inhibitor ALS-L1023 from Lemon-Balm Leaves Attenuates High-Fat Diet-Induced Nonalcoholic Fatty Liver Disease through Regulating the Visceral Adipose-Tissue Function. Int J Mol Sci 18:N/A (2017). IHC-P ; Mouse . Read more (PubMed: 28420164) »
  • Furukawa S  et al. Interleukin-33 produced by M2 macrophages and other immune cells contributes to Th2 immune reaction of IgG4-related disease. Sci Rep 7:42413 (2017). IHC ; Human . Read more (PubMed: 28205524) »

See all 93 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (stomach)
Permeabilization
Yes - 0.05% TX-100
Specification
stomach
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
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Verified customer

Submitted Aug 28 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (liver)
Permeabilization
Yes - 0.05% Triton X-100
Specification
liver
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde
Username

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Verified customer

Submitted Jun 27 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

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Verified customer

Submitted Apr 17 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
No
Specification
spleen
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% normal buffered formalin
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Verified customer

Submitted Feb 28 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate buffer pH6
Permeabilization
No
Specification
heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% Normal buffered formalin
Username

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Verified customer

Submitted Feb 07 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Specification
spleen
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% Normal buffered formalin
Username

Abcam user community

Verified customer

Submitted Jan 31 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Mitral valve, vascular interstitial cells)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Antigen Decloaker
Permeabilization
Yes - 0.2% Triton X-100
Specification
Mitral valve, vascular interstitial cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Formaldehyde
Username

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Verified customer

Submitted Aug 17 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (muscle)
Permeabilization
No
Specification
muscle
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde
Username

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Verified customer

Submitted Mar 14 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mammary Gland Tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate 10mM pH=6.0
Permeabilization
Yes - PBS with 3% Triton
Specification
Mammary Gland Tissue
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Username

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Verified customer

Submitted Aug 04 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Monkey Tissue sections (Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0 Bond Epitope Retrieval Solution 1 (ER1, Leica)
Permeabilization
No
Specification
Spleen
Blocking step
No blocking step used for 20 minute(s) · Concentration: 2.5% · Temperature: RT°C
Fixative
10% Neutral Buffered Formalin
Username

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Verified customer

Submitted Jun 01 2015

1-10 of 49 Abreviews or Q&A

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