Overview

  • Product name
    Anti-CD68 antibody [KP1], prediluted
    See all CD68 primary antibodies
  • Description
    Mouse monoclonal [KP1] to CD68, prediluted
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Cat, Human, Monkey
  • Immunogen

    Subcellular fraction of human alveolar macrophages

  • Positive control
    • Human tonsil, lymph node, or spleen tissue.
  • General notes

    ab74704 is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Tumors of lymphoid origin are usually not stained.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab125212 as a replacement.

Properties

  • Form
    Prediluted
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.5M Tris HCl, stabilizing protein, pH 7.6
  • Concentration information loading...
  • Purity
    Protein G purified
  • Primary antibody notes
    ab74704 is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Tumors of lymphoid origin are usually not stained.
  • Clonality
    Monoclonal
  • Clone number
    KP1
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab74704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

Ready to use.

Target

  • Function
    Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
  • Tissue specificity
    Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
  • Sequence similarities
    Belongs to the LAMP family.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localization
    Cell membrane and Endosome membrane. Lysosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 68 antibody
    • CD68 antibody
    • CD68 antigen antibody
    • CD68 molecule antibody
    • CD68_HUMAN antibody
    • DKFZp686M18236 antibody
    • gp11 antibody
    • Gp110 antibody
    • LAMP4 antibody
    • Macrophage antigen CD68 (microsialin) antibody
    • MACROPHAGE ANTIGEN CD68 antibody
    • Macrosialin antibody
    • SCARD1 antibody
    • Scavenger receptor class D member 1 antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human tonsil stained with ab74704 using peroxidase-conjugate and AEC chromogen. Note intense cytoplasmic staining of macrophages.

References

This product has been referenced in:
  • De Groot CJ  et al. Immunocytochemical characterization of the expression of inducible and constitutive isoforms of nitric oxide synthase in demyelinating multiple sclerosis lesions. J Neuropathol Exp Neurol 56:10-20 (1997). Read more (PubMed: 8990125) »
  • Noorman F  et al. Monoclonal antibodies against the human mannose receptor as a specific marker in flow cytometry and immunohistochemistry for macrophages. J Leukoc Biol 61:63-72 (1997). Read more (PubMed: 9000538) »

See all 4 Publications for this product

Customer reviews and Q&As

J'ai contacté le laboratoire qui m'a confirmé que la concentration d'ab74704 est 1ug/ml.

Thank you for contacting us.

This product is ready to use. Please use the antibody neat on the tissue sections. If in case the background is high, please try 1/10, 1/50 or 1/100 dilutions.

I hope this information is helpful to you...

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Thank you for contacting us.

Regarding the technique I mentioned when we spoke that utilizes the relative scarcity of isotype subclasses, the page at Dr. Cattoretti's Columbia University page has been removed but a version of it is at this I...

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Thank you for your enquiry.

I am sorry to confirm that as far as we are aware, ab74704CD68 antibody [KP1] has never been tested with samples from rabbit]. All tested species cross-reactivity information is stated on our datasheets, and these...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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