Overview

  • Product nameAnti-CD68 antibody [PG-M1]
    See all CD68 primary antibodies
  • Description
    Mouse monoclonal [PG-M1] to CD68
  • Tested applicationsSuitable for: IHC-Pmore details
    Unsuitable for: ICC or IHC-Fr
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Gaucher cells

  • Positive control
    • tonsil

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA, Tissue culture supernatant
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberPG-M1
  • IsotypeIgG3
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/40. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. (use ABC method). We suggest an incubation period of 30-60 minutes at room temperature.
  • Application notesIs unsuitable for ICC or IHC-Fr.
  • Target

    • FunctionCould play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
    • Tissue specificityHighly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
    • Sequence similaritiesBelongs to the LAMP family.
    • Post-translational
      modifications
      N- and O-glycosylated.
    • Cellular localizationCell membrane and Endosome membrane. Lysosome membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 68 antibody
      • CD68 antibody
      • CD68 antigen antibody
      • CD68 molecule antibody
      • CD68_HUMAN antibody
      • DKFZp686M18236 antibody
      • gp11 antibody
      • Gp110 antibody
      • LAMP4 antibody
      • Macrophage antigen CD68 (microsialin) antibody
      • MACROPHAGE ANTIGEN CD68 antibody
      • Macrosialin antibody
      • SCARD1 antibody
      • Scavenger receptor class D member 1 antibody
      see all

    References for Anti-CD68 antibody [PG-M1] (ab783)

    This product has been referenced in:
    • Hultman K  et al. The APOE ?4/?4 genotype potentiates vascular fibrin(ogen) deposition in amyloid-laden vessels in the brains of Alzheimer's disease patients. J Cereb Blood Flow Metab 33:1251-8 (2013). Read more (PubMed: 23652625) »
    • Horny HP  et al. Immunoreactivity of normal and neoplastic human tissue mast cells with macrophage-associated antibodies, with special reference to the recently developed monoclonal antibody PG-M1. Hum Pathol 24:355-8 (1993). Read more (PubMed: 8491475) »

    See all 4 Publications for this product

    Product Wall

    Thank you for your inquiry.

    I apologise for the delay. I had to wait for an answer from the source of these two products.

    I can now confirm that the concentration of ab783 is 10ug/ml and ab844 is approximately 2-4ug/ml.

    Th...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (granulomatous inflammatory tissue)
    Specification granulomatous inflammatory tissue
    Fixative Paraformaldehyde
    Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Pepsin
    Permeabilization No
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 21 2008

    Thank you for your enquiry regarding ab783. We have received feedback from the source of the antibody telling us that "this CD68 is specific against macrophages only" unfortunately. I would like to recommend looking at the following page from our w...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"