Anti-CD68 antibody [PG-M1] (ab783)
Key features and details
- Mouse monoclonal [PG-M1] to CD68
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG3
Overview
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Product name
Anti-CD68 antibody [PG-M1]
See all CD68 primary antibodies -
Description
Mouse monoclonal [PG-M1] to CD68 -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details
Unsuitable for: ICC or IHC-Fr -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to CD68. Gaucher cells
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Positive control
- IHC-P: Human tonsil and granulomatous inflammatory tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: 98.95% Tissue culture supernatant, 1% BSA -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
PG-M1 -
Isotype
IgG3 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab783 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (2) |
1/10 - 1/40. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. (use ABC method). We suggest an incubation period of 30-60 minutes at room temperature.
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Notes |
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IHC-P
1/10 - 1/40. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. (use ABC method). We suggest an incubation period of 30-60 minutes at room temperature. |
Target
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Function
Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. -
Tissue specificity
Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites. -
Sequence similarities
Belongs to the LAMP family. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Cell membrane and Endosome membrane. Lysosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 968 Human
- Omim: 153634 Human
- SwissProt: P34810 Human
- Unigene: 647419 Human
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Alternative names
- CD 68 antibody
- CD68 antibody
- CD68 antigen antibody
see all
Images
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ab783 staining CD68 in Human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
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Paraformaldehyde-fixed, paraffin-embedded human granulomatous inflammatory tissue stained for CD68 using ab783 in immunohistochemical analysis.
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab783 has been referenced in 15 publications.
- Wang PC et al. The spatial distribution of immune cell subpopulations in hepatocellular carcinoma. Cancer Sci 113:423-431 (2022). PubMed: 34779545
- Johnson BE et al. An omic and multidimensional spatial atlas from serial biopsies of an evolving metastatic breast cancer. Cell Rep Med 3:100525 (2022). PubMed: 35243422
- Sundaram S et al. Deciphering the Immune Complexity in Esophageal Adenocarcinoma and Pre-Cancerous Lesions With Sequential Multiplex Immunohistochemistry and Sparse Subspace Clustering Approach. Front Immunol 13:874255 (2022). PubMed: 35663986
- Yamada K et al. Siglec-7 is a predictive biomarker for the efficacy of cancer vaccination against metastatic colorectal cancer. Oncol Lett 21:10 (2021). PubMed: 33240416
- Waller R et al. Heterogeneity of cellular inflammatory responses in ageing white matter and relationship to Alzheimer's and small vessel disease pathologies. Brain Pathol 31:e12928 (2021). PubMed: 33336479