Anti-CD68 antibody [PG-M1], prediluted (ab844)


  • Product nameAnti-CD68 antibody [PG-M1], prediluted
    See all CD68 primary antibodies
  • Description
    Mouse monoclonal [PG-M1] to CD68, prediluted
  • SpecificityThis antibody reacts with a glycoprotein of 110 kDa, expressed as intracytoplasmic molecule. It stains macrophages in a wide variety of tissues. Myeloid precursor cells and peripheral granulocytes are negative. This antibody does not react with granulocytes and their precursor cells.
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    BALB/C mice were immunized with Gaucher’s cells.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: Inert stabilizer
  • Concentration information loading...
  • ClonalityMonoclonal
  • Clone numberPG-M1
  • Myelomaunknown
  • IsotypeIgG3
  • Light chain typekappa
  • Research areas


Our Abpromise guarantee covers the use of ab844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesThis antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections.
    No further titration is required.
    We suggest an incubation period of 30-60 minutes at room temperature.
    However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.
    Enzymatic predigestion with proteolytic enzymes is recommended for paraffin embedded tissue sections.
    This antibody is not recommended for frozen sections or cell smears.
  • Target

    • FunctionCould play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
    • Tissue specificityHighly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
    • Sequence similaritiesBelongs to the LAMP family.
    • Post-translational
      N- and O-glycosylated.
    • Cellular localizationCell membrane and Endosome membrane. Lysosome membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 68 antibody
      • CD68 antibody
      • CD68 antigen antibody
      • CD68 molecule antibody
      • CD68_HUMAN antibody
      • DKFZp686M18236 antibody
      • gp11 antibody
      • Gp110 antibody
      • LAMP4 antibody
      • Macrophage antigen CD68 (microsialin) antibody
      • MACROPHAGE ANTIGEN CD68 antibody
      • Macrosialin antibody
      • SCARD1 antibody
      • Scavenger receptor class D member 1 antibody
      see all

    Anti-CD68 antibody [PG-M1], prediluted images

    • ab844 staining CD68 in formalin-fixed, paraffin-embedded Human tonsil tissue by Immunohistochemistry. Staining was detected using DAB.

    References for Anti-CD68 antibody [PG-M1], prediluted (ab844)

    This product has been referenced in:
    • Tewari A  et al. Upregulation of MMP12 and its activity by UVA1 in human skin: potential implications for photoaging. J Invest Dermatol 134:2598-609 (2014). IF . Read more (PubMed: 24714202) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Spleen)
    Specification Spleen
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization No
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

    Mr. Carl Hobbs

    Verified customer

    Submitted Sep 06 2012

    Thank you for your inquiry.

    I apologise for the delay. I had to wait for an answer from the source of these two products.

    I can now confirm that the concentration of ab783 is 10ug/ml and ab844 is approximately 2-4ug/ml.


    Read More