The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.125µg for 105-8 cells. Staining the cell sample in a final volume of 100 µL is recommended
ab18418-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
FunctionCould play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
Tissue specificityHighly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites.
Sequence similaritiesBelongs to the LAMP family.
Post-translational modificationsN- and O-glycosylated.
Cellular localizationCell membrane and Endosome membrane. Lysosome membrane.
Flow cytometry staining of normal human peripheral blood cells with Anti-Mouse CD11b FITC and 0.06 µg of Mouse IgG2b ? Isotype Control Biotin (left) or 0.06 µg of ab95564 (right) followed by Streptavidin PE. Cells in the lymphocyte gate were used for analysis.
References for Anti-CD68 antibody [Y1/82A] (Biotin) (ab95564)
This product has been referenced in:
Davey FR et al. Monoclonal antibody (Y1/82A) with specificity towards peripheral blood monocytes and tissue macrophages. J Clin Pathol41:753-8 (1988).
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