Anti-CD69 antibody [FN50] (Allophycocyanin) (ab95609)

Overview

  • Product name
    Anti-CD69 antibody [FN50] (Allophycocyanin)
    See all CD69 primary antibodies
  • Description
    Mouse monoclonal [FN50] to CD69 (Allophycocyanin)
  • Conjugation
    Allophycocyanin. Ex: 645nm, Em: 660nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human, Cynomolgus monkey
  • Immunogen

    Stimulated Human B lymphocytes

  • Positive control
    • 3 day PHA activated Human peripheral blood leukocytes; Resting and 6 hour TPA activated Human PBMC
  • General notes

    Excitation: 633 nm Emission: 660 nm.

Properties

Applications

Our Abpromise guarantee covers the use of ab95609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 5µl for 106 cells. ab37391-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.
  • Tissue specificity
    Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets.
  • Sequence similarities
    Contains 1 C-type lectin domain.
  • Developmental stage
    Earliest inducible cell surface glycoprotein acquired during lymphoid activation.
  • Post-translational
    modifications
    Constitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activation inducer molecule (AIM/CD69) antibody
    • Activation inducer molecule antibody
    • AIM antibody
    • BL-AC/P26 antibody
    • BLAC/P26 antibody
    • C-type lectin domain family 2 member C antibody
    • CD69 antibody
    • CD69 antigen (p60, early T-cell activation antigen) antibody
    • CD69 antigen antibody
    • CD69 molecule antibody
    • CD69_HUMAN antibody
    • CLEC2C antibody
    • EA1 antibody
    • Early activation antigen CD69 antibody
    • Early lymphocyte activation antigen antibody
    • Early T cell activation antigen p60 antibody
    • Early T-cell activation antigen p60 antibody
    • GP32/28 antibody
    • Leu23 antibody
    • Leukocyte surface antigen Leu-23 antibody
    • MLR-3 antibody
    • MLR3 antibody
    • VEA antibody
    • Very Early Activation Antigen antibody
    see all

Images

  • Flow cytometry staining of 3-day PHA activated Human peripheral blood leukocytes with APC Mouse IgG1 kappa Isotype Control (open histogram) or ab95609 (colored histogram). Total viable cells were used for analysis.
  • Flow cytometry analysis of Cynomolgus monkey whole blood after red blood cell lysis, staining CD69 with ab95609.

    Cells were stimulated with PMA and red blood cells were lysed. Samples were fixed with paraformaldehyde. The sample was incubated with the primary antibody (1/100 in 1% BSA in PBS) for 1 hour at 4°C.

    Gating Strategy: Live lymphocytes

    See Abreview

References

ab95609 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (PBMC)
Specification
PBMC
Preparation
Cell harvesting/tissue preparation method: Ficoll-Hypaque gradient
Sample buffer: PBS
Permeabilization
No
Gating Strategy
lymphocytes
Username

Abcam user community

Verified customer

Submitted May 10 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Rat Cell (Splenocytes)
Specification
Splenocytes
Preparation
Cell harvesting/tissue preparation method: isolated from spleen of immunized rat
Sample buffer: PBS/BSA 1%
Permeabilization
No
Gating Strategy
alive lymphocytes
Username

Abcam user community

Verified customer

Submitted Jan 08 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Mouse Cell (Splenocytes)
Specification
Splenocytes
Preparation
Cell harvesting/tissue preparation method: isolated from spleen. Anti-CD3/CD28 stimulation
Sample buffer: PBS/BSA 1%
Permeabilization
No
Gating Strategy
alive lymphocytes
Username

Abcam user community

Verified customer

Submitted Jan 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (from buffy coat)
Specification
from buffy coat
Preparation
Cell harvesting/tissue preparation method: after ficoll purification and anti-CD3/CD28 overnight stimulation
Sample buffer: PBS/BSA 1%
Permeabilization
No
Gating Strategy
alive cells
Username

Abcam user community

Verified customer

Submitted Jan 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Cynomolgus Monkey Cell (from whole blood)
Specification
from whole blood
Preparation
Cell harvesting/tissue preparation method: after red blood cell lysis and PMA stimulation
Sample buffer: PBS/BSA 1%
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
alive lymphocytes
Username

Abcam user community

Verified customer

Submitted Jan 02 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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