Overview

  • Product nameAnti-CD69 antibody [H1.2F3]
    See all CD69 primary antibodies
  • Description
    Armenian Hamster monoclonal [H1.2F3] to CD69
  • Specificityab25190 recognises mouse CD69/Very Early Activation Antigen (VEA),.
  • Tested applicationsSuitable for: IHC-FoFr, Flow Cyt, IP, Celluar Activationmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation (Mouse) - Y245 mouse dendritic epidermal T cell line.

  • General notes


    This antibody augments PMA-induced T-cell proliferation, and induces redirected lysis of Fc receptor-bearing target cells by NK cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab25190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 22483694
Flow Cyt Use at an assay dependent concentration.

Pubmed:2838547 and Pubmed:2026886





ab18474-Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.

Please see: Pubmed:2838547

Celluar Activation Use at an assay dependent concentration.

T cell and NK cells. Please see: Pubmed:2838547 and Pubmed:2026886

 

Target

  • FunctionInvolved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.
  • Tissue specificityExpressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets.
  • Sequence similaritiesContains 1 C-type lectin domain.
  • Developmental stageEarliest inducible cell surface glycoprotein acquired during lymphoid activation.
  • Post-translational
    modifications
    Constitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activation inducer molecule (AIM/CD69) antibody
    • Activation inducer molecule antibody
    • AIM antibody
    • BL-AC/P26 antibody
    • BLAC/P26 antibody
    • C-type lectin domain family 2 member C antibody
    • CD69 antibody
    • CD69 antigen (p60, early T-cell activation antigen) antibody
    • CD69 antigen antibody
    • CD69 molecule antibody
    • CD69_HUMAN antibody
    • CLEC2C antibody
    • EA1 antibody
    • Early activation antigen CD69 antibody
    • Early lymphocyte activation antigen antibody
    • Early T cell activation antigen p60 antibody
    • Early T-cell activation antigen p60 antibody
    • GP32/28 antibody
    • Leu23 antibody
    • Leukocyte surface antigen Leu-23 antibody
    • MLR-3 antibody
    • MLR3 antibody
    • VEA antibody
    • Very Early Activation Antigen antibody
    see all

Anti-CD69 antibody [H1.2F3] images

  • Immunohistochemical analysis for the localization of CD69, KC and MIP-2 in the lung after lipopolysaccharide instillation. Lipopolysaccharide or PBS was administered intratracheally to WT and CD69−/− mice. Mice were sacrificed 24 h after the challenge. The lung sections from WT mice were stained with an anti-CD69 antibody followed by ABC staining method (A,phosphate-buffered saline administration; B and C, LPS administration).

    The lung sections from WT mice with LPS administration were stained with anti-CD69 in combination with biotinylated anti-KC (P) or biotinylated anti-MIP-2 (Q), followed by Alexa Fluor 594-conjugated second antibody and Alexa Fluor 488-conjugated streptavidin, respectively. All sections were stained with DAPI.

    Each scale bar represents 50 μm.

References for Anti-CD69 antibody [H1.2F3] (ab25190)

This product has been referenced in:

See 1 Publication for this product

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Thank you for your enquiry. The antibody you are looking at, ab25190, can be used for cellular activation of T and NK cells, but for cultured cells. It has not been tested in vivo. I just did a quick search on our website and there are no CD69 antibodi...

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