Tissue/ cell preparation (Mouse) - Y245 mouse dendritic epidermal T cell line.
This antibody augments PMA-induced T-cell proliferation, and induces redirected lysis of Fc receptor-bearing target cells by NK cells.
Our Abpromise guarantee covers the use of ab25190 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 22483694|
|Flow Cyt||Use at an assay dependent concentration.
Pubmed:2838547 and Pubmed:2026886
ab18474-Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.
Please see: Pubmed:2838547
|Cellular Activation||Use at an assay dependent concentration.
T cell and NK cells. Please see: Pubmed:2838547 and Pubmed:2026886
Immunohistochemical analysis for the localization of CD69, KC and MIP-2 in the lung after lipopolysaccharide instillation. Lipopolysaccharide or PBS was administered intratracheally to WT and CD69−/− mice. Mice were sacrificed 24 h after the challenge. The lung sections from WT mice were stained with an anti-CD69 antibody followed by ABC staining method (A,phosphate-buffered saline administration; B and C, LPS administration).
The lung sections from WT mice with LPS administration were stained with anti-CD69 in combination with biotinylated anti-KC (P) or biotinylated anti-MIP-2 (Q), followed by Alexa Fluor 594-conjugated second antibody and Alexa Fluor 488-conjugated streptavidin, respectively. All sections were stained with DAPI.
Each scale bar represents 50 μm.