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  • Product nameAnti-CD69 antibody [H1.2F3] (PE/Cy5®)
    See all CD69 primary antibodies
  • Description
    Armenian Hamster monoclonal [H1.2F3] to CD69 (PE/Cy5®)
  • ConjugationPE/Cy5®. Ex: 496nm, Em: 670nm
  • Tested applicationsFlow Cyt, IHC-Fr, IP, Cellular activationmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation: Y245 mouse dendritic epidermal T cell line.

  • General notesThis product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.

    This antibody augments PMA induced T cell proliferation, and induces redirected lysis of Fc receptor bearing target cells by NK cells.



Our Abpromise guarantee covers the use of ab25491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.2µg for 106 cells. Ab157805-Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.

ab157805-Armenian Hamster polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Cellular activation Use at an assay dependent concentration.

In vitro T cell and NK cell activation.


  • FunctionInvolved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.
  • Tissue specificityExpressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets.
  • Sequence similaritiesContains 1 C-type lectin domain.
  • Developmental stageEarliest inducible cell surface glycoprotein acquired during lymphoid activation.
  • Post-translational
    Constitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activation inducer molecule (AIM/CD69) antibody
    • Activation inducer molecule antibody
    • AIM antibody
    • BL-AC/P26 antibody
    • BLAC/P26 antibody
    • C-type lectin domain family 2 member C antibody
    • CD69 antibody
    • CD69 antigen (p60, early T-cell activation antigen) antibody
    • CD69 antigen antibody
    • CD69 molecule antibody
    • CD69_HUMAN antibody
    • CLEC2C antibody
    • EA1 antibody
    • Early activation antigen CD69 antibody
    • Early lymphocyte activation antigen antibody
    • Early T cell activation antigen p60 antibody
    • Early T-cell activation antigen p60 antibody
    • GP32/28 antibody
    • Leu23 antibody
    • Leukocyte surface antigen Leu-23 antibody
    • MLR-3 antibody
    • MLR3 antibody
    see all

References for Anti-CD69 antibody [H1.2F3] (PE/Cy5®) (ab25491)

This product has been referenced in:
  • Sobel ES  et al. Aberrant expression of the very early activation antigen on MRL/Mp-lpr/lpr lymphocytes. J Immunol 150:673-82 (1993). Read more (PubMed: 8380429) »
  • Karlhofer FM & Yokoyama WM Stimulation of murine natural killer (NK) cells by a monoclonal antibody specific for the NK1.1 antigen. IL-2-activated NK cells possess additional specific stimulation pathways. J Immunol 146:3662-73 (1991). Read more (PubMed: 2026886) »

See all 3 Publications for this product

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The corresponding isotype control has been added to the catalogue:

Armenian Hamster IgG (PE/Cy5®) - Isotype control, ab157805