PE/Cy7® Anti-CD69 antibody [H1.2F3] (ab25529)
Key features and details
- PE/Cy7® Armenian hamster monoclonal [H1.2F3] to CD69
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE/Cy7®. Ex: 496nm, Em: 774nm
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
PE/Cy7® Anti-CD69 antibody [H1.2F3]
See all CD69 primary antibodies -
Description
PE/Cy7® Armenian hamster monoclonal [H1.2F3] to CD69 -
Host species
Armenian hamster -
Conjugation
PE/Cy7®. Ex: 496nm, Em: 774nm -
Specificity
ab25529 recognises mouse CD69/Very Early Activation Antigen (VEA). -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse CD69. Mouse dendritic epidermal cell line Y245
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General notes
This antibody augments PMA-induced T-cell proliferation, and induces redirected lysis of Fc receptor-bearing target cells by NK cells.
This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5, 268, 486 and related patents. Cy® and CyDye® are trademarks of Cytiva.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.1
Preservative: 0.09% Sodium azide
Constituents: PBS, 16% Sucrose
Also contains a stabilizing agent. -
Concentration information loading...
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Purity
Affinity purified -
Primary antibody notes
This antibody augments PMA-induced T-cell proliferation, and induces redirected lysis of Fc receptor-bearing target cells by NK cells. -
Clonality
Monoclonal -
Clone number
H1.2F3 -
Isotype
IgG -
Light chain type
lambda -
Research areas
Associated products
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Alternative Versions
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use 0.2µg for 106 cells.
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Notes |
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Flow Cyt
Use 0.2µg for 106 cells. |
Target
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Function
Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets. -
Tissue specificity
Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets. -
Sequence similarities
Contains 1 C-type lectin domain. -
Developmental stage
Earliest inducible cell surface glycoprotein acquired during lymphoid activation. -
Post-translational
modificationsConstitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 12515 Mouse
- SwissProt: P37217 Mouse
- Unigene: 74745 Mouse
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Alternative names
- Activation inducer molecule (AIM/CD69) antibody
- Activation inducer molecule antibody
- AIM antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab25529 has not yet been referenced specifically in any publications.