Anti-CD69 antibody [H1.2F3] (PE/Cy7 ®) (ab25529)

Overview

  • Product name
    Anti-CD69 antibody [H1.2F3] (PE/Cy7 ®)
    See all CD69 primary antibodies
  • Description
    Armenian Hamster monoclonal [H1.2F3] to CD69 (PE/Cy7 ®)
  • Conjugation
    PE/Cy7 ®. Ex: 496nm, Em: 774nm
  • Specificity
    ab25529 recognises mouse CD69/Very Early Activation Antigen (VEA).
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, IP, Cellular Activationmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation (Mouse) - Y245 mouse dendritic epidermal T cell line.

  • General notes
    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.


    This antibody augments PMA-induced T-cell proliferation, and induces redirected lysis of Fc receptor-bearing target cells by NK cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab25529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.2µg for 106 cells.
IHC-Fr Use at an assay dependent dilution. Fix with acetone.
IP Use at an assay dependent dilution.
Cellular Activation Use at an assay dependent dilution. In vitro T cell and NK cell.

Target

  • Function
    Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.
  • Tissue specificity
    Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets.
  • Sequence similarities
    Contains 1 C-type lectin domain.
  • Developmental stage
    Earliest inducible cell surface glycoprotein acquired during lymphoid activation.
  • Post-translational
    modifications
    Constitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activation inducer molecule (AIM/CD69) antibody
    • Activation inducer molecule antibody
    • AIM antibody
    • BL-AC/P26 antibody
    • BLAC/P26 antibody
    • C-type lectin domain family 2 member C antibody
    • CD69 antibody
    • CD69 antigen (p60, early T-cell activation antigen) antibody
    • CD69 antigen antibody
    • CD69 molecule antibody
    • CD69_HUMAN antibody
    • CLEC2C antibody
    • EA1 antibody
    • Early activation antigen CD69 antibody
    • Early lymphocyte activation antigen antibody
    • Early T cell activation antigen p60 antibody
    • Early T-cell activation antigen p60 antibody
    • GP32/28 antibody
    • Leu23 antibody
    • Leukocyte surface antigen Leu-23 antibody
    • MLR-3 antibody
    • MLR3 antibody
    • VEA antibody
    • Very Early Activation Antigen antibody
    see all

Images

  • Flow cytometry analysis of Con-A stimulated BALB/c mouse splenocutes with ab25529. 

References

ab25529 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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