Recombinant
RabMAb

Anti-CD7 antibody [EPR4242] (ab109296)

Overview

  • Product name
    Anti-CD7 antibody [EPR4242]
    See all CD7 primary antibodies
  • Description
    Rabbit monoclonal [EPR4242] to CD7
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues on the C terminus in the intracellular domain of Human CD7.

  • Positive control
    • WB: Human tonsil, spleen and peripheral blood mononuclear cell (PBMC) lysates IHC-P: Human tonsil and colonic tissues
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Alternative versions available:

    Anti-CD7 antibody (Alexa Fluor® 488) [EPR4242] (ab199022)
    Anti-CD7 antibody (Alexa Fluor® 647) [EPR4242] (ab199023)
    Anti-CD7 antibody (HRP) [EPR4242] (ab199024)

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)
    KD = 2.40 x 10 -12 M Learn more about KD
  • Storage buffer
    PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR4242
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 25 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Not yet known.
    • Sequence similarities
      Contains 1 Ig-like (immunoglobulin-like) domain.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD7 antibody
      • CD7 antigen (p41) antibody
      • CD7 antigen antibody
      • CD7 molecule antibody
      • CD7_HUMAN antibody
      • GP40 antibody
      • LEU 9 antibody
      • LEU9 antibody
      • p41 protein antibody
      • T cell antigen CD7 antibody
      • T cell leukemia antigen antibody
      • T cell surface antigen Leu 9 antibody
      • T-cell antigen CD7 antibody
      • T-cell leukemia antigen antibody
      • T-cell surface antigen Leu-9 antibody
      • Tp 40 antibody
      • Tp40 antibody
      • TP41 antibody
      see all

    Anti-CD7 antibody [EPR4242] images

    • All lanes : Anti-CD7 antibody [EPR4242] (ab109296) at 1/1000 dilution

      Lane 1 : Human tonsil lysate
      Lane 2 : Human spleen lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 25 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    • Anti-CD7 antibody [EPR4242] (ab109296) at 1/1000 dilution + Human peripheral blood mononuclear cell (PBMC) lysate at 10 µg

      Predicted band size : 25 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of CD7 in paraffin embedded Human tonsil tissue, using ab109296 at a diluiton of 1/100.
    • Immunohistochemical analysis of CD7 in paraffin embedded Human colonic tissue, using ab109296 at a diluiton of 1/100.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-CD7 antibody [EPR4242] (ab109296)

    ab109296 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab109296.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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