Overview

  • Product nameAnti-CD7 antibody [Huly-m2] (Phycoerythrin)
    See all CD7 primary antibodies
  • Description
    Mouse monoclonal [Huly-m2] to CD7 (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • SpecificityThis antibody does not react with granulocytes and B lymphocytes.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPBS with 0.5% BSA and 0.1% sodium azide
  • Concentration information loading...
  • ClonalityMonoclonal
  • Clone numberHuly-m2
  • Myelomaunknown
  • IsotypeIgG2a
  • Light chain typeunknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notesDiagnosis and follow-up of T cell acute lymphoblastic leukemia (T-ALL) andAcute myeloid leukaemia (CD7+ AML)in human lysed whole peripheral blood or mononuclear cells separated by density gradient.
    For FACS:

    Add 100ml of whole blood to a staining tube.
    To this tube add 10ml of CD7 (PE)reagent.
    Mix gently and incubate 20-30 minutes at 2-8°C.
    Add 2ml of BIO LISANTE(ab1159) at room temperature.
    Vortex tube gently (no more then 2 seconds)then incubate for 10 minutes at room temperature in the dark.
    Wash cells two timeswith 3ml of PBS.
    Resuspend cells in 0.5-1ml of PBS and analyse.

    CD7 (PE) immunofluorescence analysis can be performed on a flow cytometerequipped with an excitation source of 488nm and fitted with logarithmic amplifiers.

    10µl ofCD7 (PE) is sufficient for labelling of 1x106 cells.
    Molecular F/P ratio: 1.0
  • Target

    • FunctionNot yet known.
    • Sequence similaritiesContains 1 Ig-like (immunoglobulin-like) domain.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD7 antibody
      • CD7 antigen (p41) antibody
      • CD7 antigen antibody
      • CD7 molecule antibody
      • CD7_HUMAN antibody
      • GP40 antibody
      • LEU 9 antibody
      • LEU9 antibody
      • p41 protein antibody
      • T cell antigen CD7 antibody
      • T cell leukemia antigen antibody
      • T cell surface antigen Leu 9 antibody
      • T-cell antigen CD7 antibody
      • T-cell leukemia antigen antibody
      • T-cell surface antigen Leu-9 antibody
      • Tp 40 antibody
      • Tp40 antibody
      • TP41 antibody
      see all

    References for Anti-CD7 antibody [Huly-m2] (Phycoerythrin) (ab1160)

    This product has been referenced in:
    • Porwit-MacDonald A  et al. BIOMED-1 concerted action report: flow cytometric characterization of CD7+ cell subsets in normal bone marrow as a basis for the diagnosis and follow-up of T cell acute lymphoblastic leukemia (T-ALL). Leukemia 14:816-25 (2000). Read more (PubMed: 10803512) »
    • Thurlow PJ  et al. A monoclonal antibody detecting a new human T cell antigen, HuLy-m2. Transplantation 38:143-7 (1984). Read more (PubMed: 6205484) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"