Anti-CD71 antibody [DF 1513 (Workshop IV)] (FITC) (ab116021)

Overview

  • Product nameAnti-CD71 antibody [DF 1513 (Workshop IV)] (FITC)
    See all CD71 primary antibodies
  • Description
    Mouse monoclonal [DF 1513 (Workshop IV)] to CD71 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsSuitable for: WB, IHC-Fr, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KG1 acute myeloid leukemia cell line.

  • Positive control
    • Human tonsil and placenta tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab116021 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/200. Detects a band of approximately 90-95 kDa (predicted molecular weight: 85 kDa).
IHC-Fr 1/50 - 1/100.
ICC Use at an assay dependent dilution. (methanol fixed).
ICC/IF 1/10 - 1/40.

Target

  • RelevanceCD71 is a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Mice that are deficient in this receptor show impaired erythroid development and abnormal iron homeostasis.
  • Cellular localizationCell Membrane.
  • Database links
  • Alternative names
    • 2610028K12Rik antibody
    • AI195355 antibody
    • AI426448 antibody
    • AU015758 antibody
    • CD71 antibody
    • CD71 antigen antibody
    • E430033M20Rik antibody
    • Mtvr-1 antibody
    • Mtvr1 antibody
    • p90 antibody
    • sTfR antibody
    • T9 antibody
    • TFR antibody
    • TFR1 antibody
    • TFRC antibody
    • TFRC transferrin receptor (p90 CD71) antibody
    • TR antibody
    • transferrin receptor antibody
    • transferrin receptor protein 1 antibody
    • Transferrin receptor protein 1, serum form antibody
    • TRFR antibody
    see all

References for Anti-CD71 antibody [DF 1513 (Workshop IV)] (FITC) (ab116021)

ab116021 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your email.

Unfortunately, no information about the use of a permeabilization step was found by the lab. Thus, it is not clear if the binding site is extra- or intracellular.

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I have heard back from the lab that the epitope of this antibody is not known. I have asked them to check if a permeabilization step is needed as this would tell us if the antibody binding site would be extracell...

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