Overview

  • Product nameAnti-CD73 antibody [10f1]
    See all CD73 primary antibodies
  • Description
    Mouse monoclonal [10f1] to CD73
  • SpecificityThis antibody reacts specifically with human 5'-nucleotidase cN-II (63kDa).
  • Tested applicationsSuitable for: WB, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human CD73.

Properties

Applications

Our Abpromise guarantee covers the use of ab59462 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/1000. Predicted molecular weight: 63 kDa.
ICC 1/200 - 1/1000.
ICC/IF Use at an assay dependent concentration. PubMed: 20601304

Target

  • FunctionHydrolyzes extracellular nucleotides into membrane permeable nucleosides.
  • Involvement in diseaseDefects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.
  • Sequence similaritiesBelongs to the 5'-nucleotidase family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5' NT antibody
    • 5' nucleotidase (CD73) antibody
    • 5' nucleotidase precursor antibody
    • 5' nucleotidase, ecto antibody
    • 5' nucleotidase, ecto (CD73) antibody
    • 5'-NT antibody
    • 5'-nucleotidase antibody
    • 5NTD_HUMAN antibody
    • CD73 antibody
    • CD73 antigen antibody
    • E5NT antibody
    • Ecto 5' nucleotidase antibody
    • Ecto-5'-nucleotidase antibody
    • eN antibody
    • eNT antibody
    • NT antibody
    • NT5 antibody
    • NT5E antibody
    • NTE antibody
    • Purine 5 Prime Nucleotidase antibody
    see all

References for Anti-CD73 antibody [10f1] (ab59462)

This product has been referenced in:
  • Kopher RA  et al. Human embryonic stem cell-derived CD34+ cells function as MSC progenitor cells. Bone 47:718-28 (2010). ICC/IF ; Human . Read more (PubMed: 20601304) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Horse Cell (Mesenchymal stem cells from umbililical cord blood)
Specification Mesenchymal stem cells from umbililical cord blood
Preparation Cell harvesting/tissue preparation method: Percoll gradient isolation from umbilical cord blood followed by selective cultivation
Sample buffer: DMEM +1% BSA
Permeabilization No
Gating Strategy based on FS/SS pattern , mesenchymal stem cells without debris
Username

Mrs. Kristel Demeyere

Verified customer

Submitted Oct 22 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"