Overview

  • Product nameAnti-CD73 antibody [1D7]
    See all CD73 primary antibodies
  • Description
    Mouse monoclonal [1D7] to CD73
  • Tested applicationsSuitable for: WB, ELISA, IHC-P, Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Purified recombinant fragment of Human CD73 expressed in E. coli.

  • Positive control
    • Human lung cancer, cholangiocarcinorna, lymph node and esophagus tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab91086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 63 kDa.
ELISA 1/10000.
IHC-P 1/200 - 1/1000.
Flow Cyt Use at an assay dependent concentration. PubMed: 23776241

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration. PubMed: 23776241

Target

  • FunctionHydrolyzes extracellular nucleotides into membrane permeable nucleosides.
  • Involvement in diseaseDefects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.
  • Sequence similaritiesBelongs to the 5'-nucleotidase family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5' NT antibody
    • 5' nucleotidase (CD73) antibody
    • 5' nucleotidase precursor antibody
    • 5' nucleotidase, ecto antibody
    • 5' nucleotidase, ecto (CD73) antibody
    • 5'-NT antibody
    • 5'-nucleotidase antibody
    • 5NTD_HUMAN antibody
    • CD73 antibody
    • CD73 antigen antibody
    • E5NT antibody
    • Ecto 5' nucleotidase antibody
    • Ecto-5'-nucleotidase antibody
    • eN antibody
    • eNT antibody
    • NT antibody
    • NT5 antibody
    • NT5E antibody
    • NTE antibody
    • Purine 5 Prime Nucleotidase antibody
    see all

Anti-CD73 antibody [1D7] images

  • ab91086, at 1/200 dilution, staining CD73 in paraffin-embedded Human lung cancer tissue by Immunohistochemistry using DAB staining.
  • ab91086, at 1/200 dilution, staining CD73 in paraffin-embedded Human cholangiocarcinorna tissue by Immunohistochemistry using DAB staining.
  • ab91086, at 1/200 dilution, staining CD73 in paraffin-embedded Human lymph node tissue by Immunohistochemistry using DAB staining.
  • ab91086, at 1/200 dilution, staining CD73 in paraffin-embedded Human esophagus tissue by Immunohistochemistry using DAB staining.

References for Anti-CD73 antibody [1D7] (ab91086)

This product has been referenced in:
  • Loi S  et al. CD73 promotes anthracycline resistance and poor prognosis in triple negative breast cancer. Proc Natl Acad Sci U S A 110:11091-6 (2013). WB, IHC-Fr, Flow Cyt ; Human . Read more (PubMed: 23776241) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH8,5
Sample Human Tissue sections (appendix)
Specification appendix
Permeabilization Yes - Washbuffer from Dako with Tween
Fixative Paraformaldehyde
Username

Mr. Rudolf Jung

Verified customer

Submitted Oct 17 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"