Overview

  • Product nameAnti-CD73 antibody [2B6]
    See all CD73 primary antibodies
  • Description
    Mouse monoclonal [2B6] to CD73
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: human CD73 (27-252aa) purified from E. coli

  • Positive control
    • Cell lysates of human mesenchymal stem cells.
  • General notesThis antibody was derived from the hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice.

Properties

Applications

Our Abpromise guarantee covers the use of ab66953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
ELISA Use at an assay dependent concentration.

Target

  • FunctionHydrolyzes extracellular nucleotides into membrane permeable nucleosides.
  • Involvement in diseaseDefects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.
  • Sequence similaritiesBelongs to the 5'-nucleotidase family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5' NT antibody
    • 5' nucleotidase (CD73) antibody
    • 5' nucleotidase precursor antibody
    • 5' nucleotidase, ecto antibody
    • 5' nucleotidase, ecto (CD73) antibody
    • 5'-NT antibody
    • 5'-nucleotidase antibody
    • 5NTD_HUMAN antibody
    • CD73 antibody
    • CD73 antigen antibody
    • E5NT antibody
    • Ecto 5' nucleotidase antibody
    • Ecto-5'-nucleotidase antibody
    • eN antibody
    • eNT antibody
    • NT antibody
    • NT5 antibody
    • NT5E antibody
    • NTE antibody
    • Purine 5 Prime Nucleotidase antibody
    see all

Anti-CD73 antibody [2B6] images

  • Anti-CD73 antibody [2B6] (ab66953) at 1/1000 dilution + lysates of human mesenchymal stem cells at 30 µg

    Secondary
    goat anti-mouse
    Developed using the ECL technique

    Predicted band size : 63 kDa
    Observed band size : 63 kDa

References for Anti-CD73 antibody [2B6] (ab66953)

ab66953 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step casein solution as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: RT°C
Sample Human Tissue sections (skin)
Specification skin
Permeabilization No
Fixative Acetone
Username

Abcam user community

Verified customer

Submitted Aug 09 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"