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Tissue/ cell preparation : human endothelial cell preparation from inflamed synovial stroma from rheumatoid arthritis patients.
ab81720 causes a reduction in CD73 expression on lymphocytes.
Our Abpromise guarantee covers the use of ab81720 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 63 kDa.|
|Flow Cyt||1/50. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
|IHC-Fr||1/50. Tissue sections were fixed in acetone.|
|Functional Studies||Use at an assay dependent concentration. Antibody 4G4 functions as an inhibitor of lymphocyte binding to HUVEC. The antibody was functionally tested by incubating the lymphocytes with the antibody before adding the lymphocytes to an EC monolayer. Furthermore the monoclonal antibody 4G4 causes a reduction in CD73 expression on lymphocytes and reduces enzyme activity.|
Immunohistochemical analysis of endometrioid endometrial adenocarcinoma tissue, labeling CD73 with ab81720. Tissue sections were fixed in 10% phosphate-buffered formalin mixed with cold acetone and incubated overnight at 4°C with ab81720. Counterstained with haematoxylin.
Image from Texidó et al, Mediators of Inflammation, 2014, 1-8, Fig. 4, doi: 10.1155/2014/120673
Immunohistochemical analysis of serous endometrial adenocarcinoma tissue, labeling CD73 with ab81720. Tissue sections were fixed in 10% phosphate-buffered formalin mixed with cold acetone and incubated overnight at 4°C with ab81720. Counterstained with haematoxylin.
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