Overview

  • Product name
    Anti-CD73 antibody [4G4]
    See all CD73 primary antibodies
  • Description
    Mouse monoclonal [4G4] to CD73
  • Tested applications
    Suitable for: WB, IP, Flow Cyt, IHC-Fr, Functional Studies, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation : human endothelial cell preparation from inflamed synovial stroma from rheumatoid arthritis patients.

  • Positive control
    • HUVEC and fresh lymphocytes.
  • General notes


    ab81720 causes a reduction in CD73 expression on lymphocytes.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 0.1% BSA, PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab81720 is purified and 0.2µM filtered.
  • Primary antibody notes
    ab81720 causes a reduction in CD73 expression on lymphocytes.
  • Clonality
    Monoclonal
  • Clone number
    4G4
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 63 kDa.
IP 1/50.
Flow Cyt 1/50. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr 1/50. Tissue sections were fixed in acetone.
Functional Studies Use at an assay dependent concentration. Antibody 4G4 functions as an inhibitor of lymphocyte binding to HUVEC. The antibody was functionally tested by incubating the lymphocytes with the antibody before adding the lymphocytes to an EC monolayer. Furthermore the monoclonal antibody 4G4 causes a reduction in CD73 expression on lymphocytes and reduces enzyme activity.
ICC/IF 1/50.

Target

  • Function
    Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
  • Involvement in disease
    Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.
  • Sequence similarities
    Belongs to the 5'-nucleotidase family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5' NT antibody
    • 5' nucleotidase (CD73) antibody
    • 5' nucleotidase precursor antibody
    • 5' nucleotidase, ecto antibody
    • 5' nucleotidase, ecto (CD73) antibody
    • 5'-NT antibody
    • 5'-nucleotidase antibody
    • 5NTD_HUMAN antibody
    • CD73 antibody
    • CD73 antigen antibody
    • E5NT antibody
    • Ecto 5' nucleotidase antibody
    • Ecto-5'-nucleotidase antibody
    • eN antibody
    • eNT antibody
    • NT antibody
    • NT5 antibody
    • NT5E antibody
    • NTE antibody
    • Purine 5 Prime Nucleotidase antibody
    see all

Images

  • ab81720 at 4 µg/ml detecting CD73 in HUVEC by Flow cytometric analysis. Blue histogram represents detection of CD73. Red and black histograms represent cells without antibody and isotype control respectively.
  • Immunohistochemical analysis of endometrioid endometrial adenocarcinoma tissue, labeling CD73 with ab81720. Tissue sections were fixed in 10% phosphate-buffered formalin mixed with cold acetone and incubated overnight at 4°C with ab81720. Counterstained with haematoxylin.

  • Anti-CD73 antibody [4G4] (ab81720) at 1/50 dilution

    Secondary
    HRP-conjugated at 1/20000 dilution

    Predicted band size : 63 kDa

    Image from Texidó et al, Mediators of Inflammation, 2014, 1-8, Fig. 4, doi: 10.1155/2014/120673

  • ICC/IF image of ab81720 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81720, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879 Dylight 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of serous endometrial adenocarcinoma tissue, labeling CD73 with ab81720. Tissue sections were fixed in 10% phosphate-buffered formalin mixed with cold acetone and incubated overnight at 4°C with ab81720. Counterstained with haematoxylin.

References

This product has been referenced in:
  • Zhang GP  et al. MicroRNA-98 regulates osteogenic differentiation of human bone mesenchymal stromal cells by targeting BMP2. J Cell Mol Med 21:254-264 (2017). Flow Cyt ; Human . Read more (PubMed: 27860183) »
  • Aliagas E  et al. High expression of ecto-nucleotidases CD39 and CD73 in human endometrial tumors. Mediators Inflamm 2014:509027 (2014). IHC ; Human . Read more (PubMed: 24707115) »

See all 10 Publications for this product

Customer reviews and Q&As

Thank you for taking time to contact us. I am sorry to hear that this antibody is not providing satisfactory results. The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality...

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Thank you for contacting us.

Effectively all of the products mentioned are suitable, and therefore are guaranteed for IHC in frozen sections in the species stated on their datasheets.

However, instead of using PFA as a fixative, I w...

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Thank you for contacting us. In general, the immunoglobulin class (IgG1, IgG2a, IgGM, etc.) and light chain sub-class (kappa or lambda) is listed on the online datasheet. When the light chain is not listed, then the light chain sub-class has not be...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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