Anti-CD73 antibody (ab115289)
Key features and details
- Rabbit polyclonal to CD73
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-CD73 antibody
See all CD73 primary antibodies -
Description
Rabbit polyclonal to CD73 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human CD73 (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: P21589 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term. -
Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
-
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab115289 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/100 - 1/500. Predicted molecular weight: 63 kDa.
|
|
IHC-P | (2) |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
WB
1/100 - 1/500. Predicted molecular weight: 63 kDa. |
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. -
Involvement in disease
Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands. -
Sequence similarities
Belongs to the 5'-nucleotidase family. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 4907 Human
- Entrez Gene: 23959 Mouse
- Omim: 129190 Human
- SwissProt: P21589 Human
- SwissProt: Q61503 Mouse
- Unigene: 153952 Human
- Unigene: 244235 Mouse
-
Alternative names
- 5' NT antibody
- 5' nucleotidase (CD73) antibody
- 5' nucleotidase precursor antibody
see all
Images
-
Immunohistochemistry image of formalin-fixed, paraffin-embedded Human placenta tissue stained with ab115289 at 10 µg followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
-
Anti-CD73 antibody (ab115289) + Mouse brain tissue lysate
Predicted band size: 63 kDa
Protocols
Datasheets and documents
-
Datasheet download
References (5)
ab115289 has been referenced in 5 publications.
- Tripathi A et al. Prognostic significance and immune correlates of CD73 expression in renal cell carcinoma. J Immunother Cancer 8:N/A (2020). PubMed: 33177176
- Meng Y et al. The metabolic syndrome alters the miRNA signature of porcine adipose tissue-derived mesenchymal stem cells. Cytometry A 93:93-103 (2018). PubMed: 28678424
- Eirin A et al. Integrated transcriptomic and proteomic analysis of the molecular cargo of extracellular vesicles derived from porcine adipose tissue-derived mesenchymal stem cells. PLoS One 12:e0174303 (2017). PubMed: 28333993
- Yu YI et al. Ecto-5'-nucleotidase expression is associated with the progression of renal cell carcinoma. Oncol Lett 9:2485-2494 (2015). PubMed: 26137095
- Pagnotta SM et al. Ensemble of gene signatures identifies novel biomarkers in colorectal cancer activated through PPAR? and TNFa signaling. PLoS One 8:e72638 (2013). IHC . PubMed: 24133572