Overview

  • Product name
  • Description
    Rabbit polyclonal to CD73
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH corrresponding to a region from the C-terminal region of Human CD73 protein

  • Positive control
    • Human placenta tissue and mouse brain tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab115289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Predicted molecular weight: 63 kDa.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
  • Involvement in disease
    Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.
  • Sequence similarities
    Belongs to the 5'-nucleotidase family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5' NT antibody
    • 5' nucleotidase (CD73) antibody
    • 5' nucleotidase precursor antibody
    • 5' nucleotidase, ecto antibody
    • 5' nucleotidase, ecto (CD73) antibody
    • 5'-NT antibody
    • 5'-nucleotidase antibody
    • 5NTD_HUMAN antibody
    • CD73 antibody
    • CD73 antigen antibody
    • E5NT antibody
    • Ecto 5' nucleotidase antibody
    • Ecto-5'-nucleotidase antibody
    • eN antibody
    • eNT antibody
    • NT antibody
    • NT5 antibody
    • NT5E antibody
    • NTE antibody
    • Purine 5 Prime Nucleotidase antibody
    see all

Images

  • Immunohistochemistry image of formalin-fixed, paraffin-embedded Human placenta tissue stained with ab115289 at 10 µg followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Anti-CD73 antibody (ab115289) + Mouse brain tissue lysate

    Predicted band size : 63 kDa

References

This product has been referenced in:
  • Eirin A  et al. Integrated transcriptomic and proteomic analysis of the molecular cargo of extracellular vesicles derived from porcine adipose tissue-derived mesenchymal stem cells. PLoS One 12:e0174303 (2017). Read more (PubMed: 28333993) »
  • Yu YI  et al. Ecto-5'-nucleotidase expression is associated with the progression of renal cell carcinoma. Oncol Lett 9:2485-2494 (2015). Read more (PubMed: 26137095) »

See all 3 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab115289.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up