The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 5µl for 106 cells.
ab37391 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
Involvement in disease
Defects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.
Flow Cytometric analysis of normal Human peripheral blood cells labeling CD73 with ab155378 (purple histogram) at 0.125 µg/test in 100 µl final volume or an equal amount of Mouse IgG1 kappa Isotype Control APC (blue histogram). Cells in the lymphocyte gate were used for analysis.