Overview

  • Product nameAnti-CD73 antibody - N-terminal
    See all CD73 primary antibodies
  • Description
    Rabbit polyclonal to CD73 - N-terminal
  • Tested applicationsELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from the N-terminal region of Human CD73, conjugated to KLH

  • Positive control
    • Y79 cell lysate; human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab71322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
IHC-P 1/50 - 1/100.

Target

  • FunctionHydrolyzes extracellular nucleotides into membrane permeable nucleosides.
  • Involvement in diseaseDefects in NT5E are the cause of calcification of joints and arteries (CAJA) [MIM:211800]. A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands.
  • Sequence similaritiesBelongs to the 5'-nucleotidase family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5' NT antibody
    • 5' nucleotidase (CD73) antibody
    • 5' nucleotidase precursor antibody
    • 5' nucleotidase, ecto antibody
    • 5' nucleotidase, ecto (CD73) antibody
    • 5'-NT antibody
    • 5'-nucleotidase antibody
    • 5NTD_HUMAN antibody
    • CD73 antibody
    • CD73 antigen antibody
    • E5NT antibody
    • Ecto 5' nucleotidase antibody
    • Ecto-5'-nucleotidase antibody
    • eN antibody
    • eNT antibody
    • NT antibody
    • NT5 antibody
    • NT5E antibody
    • NTE antibody
    • Purine 5 Prime Nucleotidase antibody
    see all

Anti-CD73 antibody - N-terminal images

  • Anti-CD73 antibody - N-terminal (ab71322) at 1/100 dilution + Y79 cell lysate at 35 µg

    Predicted band size : 63 kDa
    Observed band size : 63 kDa
  • Analysis of human breast carcinoma tissue using 1:50 ab71322. DAB staining.

References for Anti-CD73 antibody - N-terminal (ab71322)

This product has been referenced in:
  • Lu XX  et al. Expression and clinical significance of CD73 and hypoxia-inducible factor-1a in gastric carcinoma. World J Gastroenterol 19:1912-8 (2013). IHC-P ; Human . Read more (PubMed: 23569336) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Deer Cell lysate - whole cell (Liver)
Loading amount 20 µg
Specification Liver
Gel Running Conditions Reduced Denaturing (12% acrylamide)
Blocking step BSA as blocking agent for 1 hour(s) and 10 minute(s) · Concentration: 5% · Temperature: 26°C
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Submitted May 11 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"