Anti-CD74 antibody [CerCLIP.1] (ab22606)

Overview

  • Product nameAnti-CD74 antibody [CerCLIP.1]
    See all CD74 primary antibodies
  • Description
    Mouse monoclonal [CerCLIP.1] to CD74
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Cow, Human, Pig
  • Immunogen

    Synthetic peptide:

    LTVTSQNLQLENLRMKLPKPPKPV

    , corresponding to amino acids 81-104 of Human CD74.

  • General notes


    CLIP or the class II associated invariant chain peptide region of the invariant chain (Ii) has several important functions in the MHC class II antigen processing pathway. In specialized antigen presenting cells (APCs) such as macrophages, B cells and dendritic cells the MHC class II alpha-beta dimers assemble in the endoplasmic reticulum (ER) with a trimer of the nonpolymorphic invariant chain to form a nonameric complex which is transported through the Golgi apparatus to the trans Golgi network. In the trans Golgi network, signals to the invariant chain cytosolic tail target the complex to organelles in the endocytic pathway. Here the peptides derived from antigens, which are usually internalized into the endocytic pathway of antigenpresenting cells, are prevented from binding MHC class II molecules which are at the early stages of their bioynthesis. This prevention is mediated by the membrane proximal domain of Ii which is degraded upon arrival in the endocytic structures prior to peptide loading and these fragments are termed CLIP which consists of amino acids 81 to 104 of Ii.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein G purified
  • Primary antibody notesCLIP or the class II associated invariant chain peptide region of the invariant chain (Ii) has several important functions in the MHC class II antigen processing pathway. In specialized antigen presenting cells (APCs) such as macrophages, B cells and dendritic cells the MHC class II alpha-beta dimers assemble in the endoplasmic reticulum (ER) with a trimer of the nonpolymorphic invariant chain to form a nonameric complex which is transported through the Golgi apparatus to the trans Golgi network. In the trans Golgi network, signals to the invariant chain cytosolic tail target the complex to organelles in the endocytic pathway. Here the peptides derived from antigens, which are usually internalized into the endocytic pathway of antigenpresenting cells, are prevented from binding MHC class II molecules which are at the early stages of their bioynthesis. This prevention is mediated by the membrane proximal domain of Ii which is degraded upon arrival in the endocytic structures prior to peptide loading and these fragments are termed CLIP which consists of amino acids 81 to 104 of Ii.
  • ClonalityMonoclonal
  • Clone numberCerCLIP.1
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab22606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionPlays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.
  • Sequence similaritiesContains 1 thyroglobulin type-1 domain.
  • Cellular localizationCell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network. Endosome. Lysosome. Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 74 antibody
    • CD74 antibody
    • CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody
    • CD74 antigen antibody
    • CD74 molecule antibody
    • CD74 molecule, major histocompatibility complex, class II invariant chain antibody
    • CLIP antibody
    • DHLAG antibody
    • Gamma chain of class II antigens antibody
    • HG2A_HUMAN antibody
    • HLA class II histocompatibility antigen gamma chain antibody
    • HLA DR antigens associated invariant chain antibody
    • HLA DR gamma antibody
    • HLA-DR antigens-associated invariant chain antibody
    • HLA-DR-gamma antibody
    • HLADG antibody
    • HLADR antigens associated invariant chain antibody
    • Ia antigen associated invariant chain antibody
    • Ia antigen-associated invariant chain antibody
    • Ia associated invariant chain antibody
    • Ia gamma antibody
    • Ii antibody
    • Invariant polypeptide of major histocompatibility complex class II antigen associated antibody
    • la-gamma antibody
    • Major histocompatibility complex class II invariant chain antibody
    • MHC HLA DR gamma chain antibody
    • MHC HLA-DR gamma chain antibody
    • p33 antibody
    • p35 antibody
    • Protein 41 antibody
    see all

References for Anti-CD74 antibody [CerCLIP.1] (ab22606)

ab22606 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (B cell Lymphoma cell lines Raji and Daudi)
Specification B cell Lymphoma cell lines Raji and Daudi
Preparation Cell harvesting/tissue preparation method: Trypsinsation and scraping
Sample buffer: PBS
Fixation Methanol
Permeabilization No
Gating Strategy Predominant population
Username

Prof. Paul Townsend

Verified customer

Submitted May 07 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"