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A synthetic peptide corresponding to residues in Human CD74.
This product is a recombinant rabbit monoclonal antibody.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Our Abpromise guarantee covers the use of ab108393 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 34 kDa.|
|IHC-P||1/100 - 1/250. Perform antigen retrieval before commencing with IHC staining protocol.|
Confocal image of ab108393 staining CD74 in the RAMOS (human Burkitt's lymphoma) cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. Samples were incubated with primary antibody (1/500) and an Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (ab150077) was used as the secondary antibody at a dilution of 1/1000.
DAPI was used ad a nuclear counterstain at 1/200.
ab108393 staining CD74 in RAMOS (human Burkitt's lymphoma) cell line. The sample was fixed with 4% paraformaldehyde and incubated with the primary antibody (1/150) for 30 minutes at 4°C. An Alexa Fluor® 488 -conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody.
Control: Cell without incubation with primary antibody and secondary antibody (Blue).
ab108393 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"