Anti-CD74 antibody [LN2] (FITC) (ab82432)

Overview

  • Product nameAnti-CD74 antibody [LN2] (FITC)
    See all CD74 primary antibodies
  • Description
    Mouse monoclonal [LN2] to CD74 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityHuman CD74
  • Tested applicationsSuitable for: IP, IHC-Fr, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    The details of the immunogen for this antibody are not available

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone numberLN2
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab82432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use 20µl for 106 cells. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionPlays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.
  • Sequence similaritiesContains 1 thyroglobulin type-1 domain.
  • Cellular localizationCell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network. Endosome. Lysosome. Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 74 antibody
    • CD74 antibody
    • CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody
    • CD74 antigen antibody
    • CD74 molecule antibody
    • CD74 molecule, major histocompatibility complex, class II invariant chain antibody
    • CLIP antibody
    • DHLAG antibody
    • Gamma chain of class II antigens antibody
    • HG2A_HUMAN antibody
    • HLA class II histocompatibility antigen gamma chain antibody
    • HLA DR antigens associated invariant chain antibody
    • HLA DR gamma antibody
    • HLA-DR antigens-associated invariant chain antibody
    • HLA-DR-gamma antibody
    • HLADG antibody
    • HLADR antigens associated invariant chain antibody
    • Ia antigen associated invariant chain antibody
    • Ia antigen-associated invariant chain antibody
    • Ia associated invariant chain antibody
    • Ia gamma antibody
    • Ii antibody
    • Invariant polypeptide of major histocompatibility complex class II antigen associated antibody
    • la-gamma antibody
    • Major histocompatibility complex class II invariant chain antibody
    • MHC HLA DR gamma chain antibody
    • MHC HLA-DR gamma chain antibody
    • p33 antibody
    • p35 antibody
    • Protein 41 antibody
    see all

Anti-CD74 antibody [LN2] (FITC) images

  • Overlay histogram showing RAMOS cells stained with ab82432 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82432, 2µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 FITC (2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References for Anti-CD74 antibody [LN2] (FITC) (ab82432)

ab82432 has not yet been referenced specifically in any publications.

Product Wall

I apologize for the confusion. The concentration is lot specific. I assume you have lot 972107 GR4796-1? This has the concentration of 0.1mg/ml.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"