Anti-CD74 antibody [MRC OX3] (ab22348)


  • Product nameAnti-CD74 antibody [MRC OX3]
    See all CD74 primary antibodies
  • Description
    Mouse monoclonal [MRC OX3] to CD74
  • SpecificityRecognises a polymorphic determinant of rat Ia antigen (RT1.Bu) present on Lewis, Wistar and AO strain rats but not BN, DA or PVG/c strains. The antibody cross reacts with mouse strains (MHC haplotypes b and s) and analysis of recombinant mouse strains showed that the determinants mapped to the I-A region. It recognises Ia antigens on B-cells, dendritic cells and certain epithelial cells. It is particularly useful for distinguishing Ia positive cells from different rat strains, eg for recognising cells of donor origin in bone marrow reconstituted radiation chimeras.
  • Tested applicationsSuitable for: IHC-R, IHC-P, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Rat thymocyte membrane glycoproteins


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesPurified IgG prepared by ion exchange chromatography from tissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberMRC OX3
  • MyelomaNS1
  • IsotypeIgG1
  • Research areas


Our Abpromise guarantee covers the use of ab22348 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-R Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • FunctionPlays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.
  • Sequence similaritiesContains 1 thyroglobulin type-1 domain.
  • Cellular localizationCell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network. Endosome. Lysosome. Transits through a number of intracellular compartments in the endocytic pathway. It can either undergo proteolysis or reach the cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 74 antibody
    • CD74 antibody
    • CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) antibody
    • CD74 antigen antibody
    • CD74 molecule antibody
    • CD74 molecule, major histocompatibility complex, class II invariant chain antibody
    • CLIP antibody
    • DHLAG antibody
    • Gamma chain of class II antigens antibody
    • HG2A_HUMAN antibody
    • HLA class II histocompatibility antigen gamma chain antibody
    • HLA DR antigens associated invariant chain antibody
    • HLA DR gamma antibody
    • HLA-DR antigens-associated invariant chain antibody
    • HLA-DR-gamma antibody
    • HLADG antibody
    • HLADR antigens associated invariant chain antibody
    • Ia antigen associated invariant chain antibody
    • Ia antigen-associated invariant chain antibody
    • Ia associated invariant chain antibody
    • Ia gamma antibody
    • Ii antibody
    • Invariant polypeptide of major histocompatibility complex class II antigen associated antibody
    • la-gamma antibody
    • Major histocompatibility complex class II invariant chain antibody
    • MHC HLA DR gamma chain antibody
    • MHC HLA-DR gamma chain antibody
    • p33 antibody
    • p35 antibody
    • Protein 41 antibody
    see all

References for Anti-CD74 antibody [MRC OX3] (ab22348)

ab22348 has not yet been referenced specifically in any publications.

Product Wall

We do not have data that answers your questions but the following paper should contain information about the production and characterization of the OX3 clone. I do not have access to the journal to confirm.

McMaster, W. R. & Williams, A. F. ...

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The antibody does not require antigen retrieval. The rest of the protocol is standard, and can be found on our website at the following link.

For a secondary antibody, ...

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Yes, "IHC-P" refers to immunohistochemistry of formalin-fixed, paraffin-embedded tissue.

Regarding mouse reactivity, it has been confirmed that the antibody reacts with mouse strains (MHC haplotypes b and s).

If you are using a se...

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