Overview

  • Product name
    Anti-CD75 antibody
    See all CD75 primary antibodies
  • Description
    Rabbit polyclonal to CD75
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Full length Human CD75 (NP_775324.1, 1 aa - 175 aa).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Mouse liver tissue lysate; CD75 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab103472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 47 kDa.

Target

  • Function
    Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates.
  • Pathway
    Protein modification; protein glycosylation.
  • Sequence similarities
    Belongs to the glycosyltransferase 29 family.
  • Post-translational
    modifications
    The soluble form derives from the membrane form by proteolytic processing.
    The HB-6, CDW75, and CD76 differentiation antigens are cell-surface carbohydrate determinants generated by this enzyme.
  • Cellular localization
    Golgi apparatus > Golgi stack membrane. Secreted. Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.
  • Information by UniProt
  • Database links
    • Alternative names
      • 6 sialyltransferase antibody
      • 6-sialyltransferase 1 antibody
      • 6-ST 1 antibody
      • Alpha 2 antibody
      • Alpha 2,6 ST 1 antibody
      • Alpha 2,6 ST antibody
      • B cell antigen CD75 antibody
      • B-cell antigen CD75 antibody
      • Beta galactoside alpha 2,6 sialyltransferase 1 antibody
      • Beta-galactoside alpha-2 antibody
      • CMP N acetylneuraminate beta galactosamide alpha 2,6 sialyltransferase 1 antibody
      • CMP-N-acetylneuraminate-beta-galactosamide-alpha-2 antibody
      • MGC48859 antibody
      • Sialyltransferase 1 (beta galactoside alpha 2,6 sialyltransferase) antibody
      • Sialyltransferase 1 antibody
      • SIAT1 antibody
      • SIAT1_HUMAN antibody
      • ST6 beta galactosamide alpha 2,6 sialyltranferase 1 antibody
      • ST6Gal I antibody
      • ST6GAL1 antibody
      • ST6GalI antibody
      • ST6N antibody
      see all

    Images

    • Western blot - Anti-CD75 antibody (ab103472)
      Western blot - Anti-CD75 antibody (ab103472)
      Anti-CD75 antibody (ab103472) at 5 µg/ml + Mouse liver tissue lysate at 50 µg

      Predicted band size: 47 kDa

    • Western blot - Anti-CD75 antibody (ab103472)
      Western blot - Anti-CD75 antibody (ab103472)
      All lanes : Anti-CD75 antibody (ab103472) at 5 µg/ml

      Lane 1 : CD75 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Predicted band size: 47 kDa



      Predicted MWt of 175 amino acid NP_775324.1 is 20.80 kDa.

    References

    ab103472 has not yet been referenced specifically in any publications.

    Publishing research using ab103472? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1 Review or Q&A

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    Votes: Highest First

    Western blot abreview for Anti-CD75 antibody

     Poor
    Abreviews
    Application
    Western blot
    Sample
    Mouse Cell lysate - other (Transfected CHO cells)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    20 µg
    Specification
    Transfected CHO cells
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
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    Verified customer

    Submitted May 03 2018

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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