Overview

  • Product name
  • Description
    Rabbit polyclonal to CD75
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to a region within internal amino acids of Human CD75.

  • Positive control
    • RAW264.7 cell extract, Human Kidney tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS is without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 47 kDa.
ELISA 1/40000.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function
    Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates.
  • Pathway
    Protein modification; protein glycosylation.
  • Sequence similarities
    Belongs to the glycosyltransferase 29 family.
  • Post-translational
    modifications
    The soluble form derives from the membrane form by proteolytic processing.
    The HB-6, CDW75, and CD76 differentiation antigens are cell-surface carbohydrate determinants generated by this enzyme.
  • Cellular localization
    Golgi apparatus > Golgi stack membrane. Secreted. Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.
  • Information by UniProt
  • Database links
  • Alternative names
    • 6 sialyltransferase antibody
    • 6-sialyltransferase 1 antibody
    • 6-ST 1 antibody
    • Alpha 2 antibody
    • Alpha 2,6 ST 1 antibody
    • Alpha 2,6 ST antibody
    • B cell antigen CD75 antibody
    • B-cell antigen CD75 antibody
    • Beta galactoside alpha 2,6 sialyltransferase 1 antibody
    • Beta-galactoside alpha-2 antibody
    • CMP N acetylneuraminate beta galactosamide alpha 2,6 sialyltransferase 1 antibody
    • CMP-N-acetylneuraminate-beta-galactosamide-alpha-2 antibody
    • MGC48859 antibody
    • Sialyltransferase 1 (beta galactoside alpha 2,6 sialyltransferase) antibody
    • Sialyltransferase 1 antibody
    • SIAT1 antibody
    • SIAT1_HUMAN antibody
    • ST6 beta galactosamide alpha 2,6 sialyltranferase 1 antibody
    • ST6Gal I antibody
    • ST6GAL1 antibody
    • ST6GalI antibody
    • ST6N antibody
    see all

Anti-CD75 antibody images

  • All lanes : Anti-CD75 antibody (ab124407) at 1/500 dilution

    Lane 1 : RAW264.7 cell extract
    Lane 2 : RAW264.7 cell extract with synthesized peptide


    Predicted band size : 47 kDa
  • Staining of CD75 in Paraffin-embedded Human kidney tissue by Immunohistochemistry using ab124407 at a concentration of 5 µg/ml.

References for Anti-CD75 antibody (ab124407)

ab124407 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
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Submitted Mar 28 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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