Anti-CD75 antibody [B-L5] (ab27316)


  • Product name
    Anti-CD75 antibody [B-L5]
    See all CD75 primary antibodies
  • Description
    Mouse monoclonal [B-L5] to CD75
  • Specificity
    This antibody recognises CD75.
  • Tested applications
    Suitable for: Flow Cyt, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Raji cell line.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purification notes
    This antibody was purified by gel filtration.
  • Clonality
  • Clone number
  • Myeloma
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab27316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: Use 10 µl to label 106 cells or 100 µl of whole blood.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates.
    • Pathway
      Protein modification; protein glycosylation.
    • Sequence similarities
      Belongs to the glycosyltransferase 29 family.
    • Post-translational
      The soluble form derives from the membrane form by proteolytic processing.
      The HB-6, CDW75, and CD76 differentiation antigens are cell-surface carbohydrate determinants generated by this enzyme.
    • Cellular localization
      Golgi apparatus > Golgi stack membrane. Secreted. Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.
    • Information by UniProt
    • Database links
    • Alternative names
      • 6 sialyltransferase antibody
      • 6-sialyltransferase 1 antibody
      • 6-ST 1 antibody
      • Alpha 2 antibody
      • Alpha 2,6 ST 1 antibody
      • Alpha 2,6 ST antibody
      • B cell antigen CD75 antibody
      • B-cell antigen CD75 antibody
      • Beta galactoside alpha 2,6 sialyltransferase 1 antibody
      • Beta-galactoside alpha-2 antibody
      • CMP N acetylneuraminate beta galactosamide alpha 2,6 sialyltransferase 1 antibody
      • CMP-N-acetylneuraminate-beta-galactosamide-alpha-2 antibody
      • MGC48859 antibody
      • Sialyltransferase 1 (beta galactoside alpha 2,6 sialyltransferase) antibody
      • Sialyltransferase 1 antibody
      • SIAT1 antibody
      • SIAT1_HUMAN antibody
      • ST6 beta galactosamide alpha 2,6 sialyltranferase 1 antibody
      • ST6Gal I antibody
      • ST6GAL1 antibody
      • ST6GalI antibody
      • ST6N antibody
      see all

    Anti-CD75 antibody [B-L5] images

    • CD75 was immunoprecipitated using 0.5mg HepG2 whole cell extract, 5µg of Mouse monoclonal to CD75 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

      The antibody was incubated under agitation with Protein G beads for 10min, HepG2 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

      Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab27316.

      Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.

      Band: 47kDa; CD75

    • Ab27316, at a dilution of 10µl for 106 cells or 100 µl of whole blood, staining CD75 by Flow Cytometry.

    References for Anti-CD75 antibody [B-L5] (ab27316)

    ab27316 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us. ab99597 is an allophycocyanin conjugated secondary antibody specific to primary Mouse IgM, it would therefore recognise ab27316 (a Mouse IgM). It would not recognise IgG. For more details about secondary antibodies pl...

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