Overview

  • Product name
    Anti-CD77 antibody [38-13]
  • Description
    Rat monoclonal [38-13] to CD77
  • Specificity
    CD77 is specific for Burkitt's lymphoma associated antigen (BLA), which is a neutral glycosphingolipid of the globoseries: globotriaosylceramide Gb3. Reacts with red blood cells of phenotype pk1 or pk2 and activated B cells in agglutination assay at 4°C and the radioactive Coombs test. Does not react with P or P2 red cells, slightly agglutinate P1 red cells after papain treatment (anti pk activity).
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue, cells or virus. Immunogen: Daudi Cell Line (Burkitt's Lymphoma)

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

Properties

Applications

Our Abpromise guarantee covers the use of ab19795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt 1/10.

ab35768 - Rat monoclonal IgM, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.

Images

  • Overlay histogram showing RAMOS cells stained with ab19795 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then incubated in 1X PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19795, 1/10 dilution) for 30 min at 22ºC. The secondary antibody used was Goat Anti-Rat IgM mu chain (DyLight® 488) preadsorbed at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat kappa monoclonal [RTK2118] (ab35768, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References

This product has been referenced in:
  • Lakomá J  et al. Increased expression of Trpv1 in peripheral terminals mediates thermal nociception in Fabry disease mouse model. Mol Pain 12:N/A (2016). ICC ; Mouse . Read more (PubMed: 27531673) »
  • Song HY  et al. Using CRISPR/Cas9-Mediated GLA Gene Knockout as an In Vitro Drug Screening Model for Fabry Disease. Int J Mol Sci 17:N/A (2016). IF . Read more (PubMed: 27983599) »

See all 5 Publications for this product

Customer reviews and Q&As

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The laboratory that supplies this to us does not check the concentration of the antibody solution, so matching an isotype control concentration to the concentration of the antibody will not be possible, unless yo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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