Overview

  • Product nameAnti-CD79a antibody [HM47/A9]See all CD79a primary antibodies ...
  • Description
    Mouse monoclonal [HM47/A9] to CD79a
  • SpecificityThis antibody labels precursor B-cell acute lymphoblastic leukemia samples, making it the most reliable B-cell marker for this disorder. In a study by Mason, et al on a total of 454 paraffin-embedded tissues, it reacted with 97% of B-cell neoplasms. We have data to indicate that this antibody may not cross react with Opossum. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsICC/IF, IHC-P, WB, Flow Cyt more details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Pig, Monkey
    Predicted to work with: Rabbit, Horse, Chicken, Guinea pig
  • Immunogen

    Synthetic peptide: GTYQDVGSLNIADVQ, corresponding to C terminal amino acids 208-222 of Human CD79a.

  • Positive control
    • Raji cells. Lymph node. Tonsil.

Properties

Applications

Our Abpromise guarantee covers the use of ab3121 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 50 kDa.
Flow Cyt Use 0.01µg for 106 cells.

Target

  • FunctionRequired in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B cells.
  • Tissue specificityB-cells.
  • Involvement in diseaseDefects in CD79A are the cause of agammaglobulinemia type 3 (AGM3) [MIM:613501]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life. Note=Two different mutations, one at the splice donor site of intron 2 and the other at the splice acceptor site for exon 3, have been identified. Both mutations give rise to a truncated protein.
  • Sequence similaritiesContains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 ITAM domain.
  • Post-translational
    modifications
    Phosphorylated on tyrosine, serine and threonine residues upon B-cell activation. Phosphorylation of tyrosine residues by Src-family kinases is an early and essential feature of the BCR signaling cascade. The phosphorylated tyrosines serve as docking sites for SH2-domain containing kinases, leading to their activation which in turn leads to phosphorylation of downstream targets. Phosphorylation of serine and threonine residues may prevent subsequent tyrosine phosphorylation.
  • Cellular localizationCell membrane. Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts.
  • Target information above from: UniProt accession P11912 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • B cell antigen receptor complex associated protein alpha chain antibody
    • B cell antigen receptor complex associated protein alpha chain precursor antibody
    • B Lymphocyte Specific MB1 Protein antibody
    • B lymphocyte-specific MB1 protein antibody
    • B-cell antigen receptor complex-associated protein alpha chain antibody
    • CD 79a antibody
    • CD79a antibody
    • CD79a antigen (immunoglobulin-associated alpha) antibody
    • CD79A antigen antibody
    • CD79A antigen immunoglobulin associated alpha antibody
    • CD79a molecule immunoglobulin associated alpha antibody
    • CD79a molecule, immunoglobulin-associated alpha antibody
    • CD79A_HUMAN antibody
    • Ig alpha antibody
    • Ig-alpha antibody
    • IGA antibody
    • IgM-alpha antibody
    • Immunoglobulin Associated Alpha antibody
    • Immunoglobulin-associated alpha antibody
    • Ly54 antibody
    • MB 1 antibody
    • MB 1 membrane glycoprotein antibody
    • MB-1 membrane glycoprotein antibody
    • MB1 antibody
    • MB1 membrane glycoprotein antibody
    • Membrane bound immunoglobulin associated protein antibody
    • Membrane-bound immunoglobulin-associated protein antibody
    • Surface IgM associated protein antibody
    • Surface IgM-associated protein antibody
    see all

Anti-CD79a antibody [HM47/A9] images

  • Human peripheral blood lymphocytes stained with ab3121 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab3121, 0.01μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

  • ab3121 at 1/200 staining rat lymphoid tissues sections (Spleen, Lymph Node, Payers Patch) by IHC-P. The tissue was formaldehyde fixed and blocked before incubation with the antibody for 1 hour. A rabbit anti-mouse antibody was used as the secondary.

    See Abreview

  • ab3121 staining human tonsil sections by IHC-P. The tissue was formaldehyde fixed and blocked before incubation for 10 minutes with 1%BSA, followed by incubations with ab3121 at 1/1000for 1h . A biotinylated goat anti-mouse polyclonal antibody at 1/200 was used as the secondary.

    See Abreview

  • ab3121 staining monkey spleen sections by IHC-P. The tissue was formaldehyde fixed and blocked before incubation for 10 minutes with 1%BSA, followed by incubations with ab3121 at 1/1000for 1h. A biotinylated goat anti-mouse polyclonal antibody at 1/200 was used as the secondary.

    See Abreview

References for Anti-CD79a antibody [HM47/A9] (ab3121)

This product has been referenced in:
  • Pero R  et al. POZ-, AT hook-, and Zinc finger-containing protein (PATZ) interacts with the human oncogene B-cell lymphoma 6 (BCL6) and is required for its negative autoregulation. J Biol Chem : (2012). IHC-P . Read more (PubMed: 22493480) »
  • Drukker M  et al. Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells. Nat Biotechnol 30:531-42 (2012). Human . Read more (PubMed: 22634564) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Antibody diluent as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH6
Sample Rat Tissue sections (Spleen)
Specification Spleen
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted May 31 2013

Thank you for your enquiry. I am pleased to provide the following suggested positive controls for these antibodies. I have mainly chosen tissue that has been tested succesfully and shown in an image on the datasheets: 1.Anti-ErbB 2 antibody (ab2428) Hu...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Tonsil)
Specification Tonsil
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Jun 02 2011

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Monkey Tissue sections (Marmoset spleen)
Specification Marmoset spleen
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Jun 02 2011

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Spleen)
Specification Spleen
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Jun 02 2011

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (brain)
Specification brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer X 1 pH6
Permeabilization No
Blocking step peroxidase blocking reagent (hydrogen peroxide and 0.031 mol/L sodium azide) and purified casein as blocking agent for 10 minute(s) · Concentration: 000% · Temperature: 25°C
Username

Mr. osama mohsen

Verified customer

Submitted Feb 19 2008

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Lymphoid Tissues (Spleen, Lymph Node, Payers Patch)
Specification Lymphoid Tissues (Spleen, Lymph Node, Payers Patch
Fixative Formaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Dako Protein Block as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jun 26 2007

Thank you for your enquiry. To our knowledge this antibody recognizes both normal and neoplastic B cells. Please read through the online datasheet for further detailed information regarding this antibody. If you require further information to that prov...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"