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Synthetic peptide: GTYQDVGSLNIADVQ, corresponding to C terminal amino acids 208-222 of Human CD79a.
Our Abpromise guarantee covers the use of ab3121 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 50 kDa.|
|Flow Cyt||Use 0.01µg for 106 cells.|
Human peripheral blood lymphocytes stained with ab3121 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab3121, 0.01μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.
ab3121 staining human tonsil sections by IHC-P. The tissue was formaldehyde fixed and blocked before incubation for 10 minutes with 1%BSA, followed by incubations with ab3121 at 1/1000for 1h . A biotinylated goat anti-mouse polyclonal antibody at 1/200 was used as the secondary.
ab3121 staining monkey spleen sections by IHC-P. The tissue was formaldehyde fixed and blocked before incubation for 10 minutes with 1%BSA, followed by incubations with ab3121 at 1/1000for 1h. A biotinylated goat anti-mouse polyclonal antibody at 1/200 was used as the secondary.
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