Anti-CD79a antibody [HM57] (ab62650)

Overview

  • Product name
    Anti-CD79a antibody [HM57]
    See all CD79a primary antibodies
  • Description
    Mouse monoclonal [HM57] to CD79a
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Human, Pig, Monkey
  • Immunogen

    Synthetic peptide:

    ISRGLQGTYQDVGSL

    , corresponding to amino acids 202-216 of Human CD79a

  • Positive control
    • Human tonsil tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab62650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
IHC-Fr
Flow Cyt
  • Application notes
    Flow Cyt: 1/10 - 1/50. Use 10µl to label 1x106 cells in 100µl. Membrane permeabilisation is required for this application.
    IHC-P: 1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Sodium citrate buffer pH 6.0 is recommended for this purpose.
    IHC-Fr: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 25 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B cells.
    • Tissue specificity
      B-cells.
    • Involvement in disease
      Defects in CD79A are the cause of agammaglobulinemia type 3 (AGM3) [MIM:613501]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life. Note=Two different mutations, one at the splice donor site of intron 2 and the other at the splice acceptor site for exon 3, have been identified. Both mutations give rise to a truncated protein.
    • Sequence similarities
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 ITAM domain.
    • Post-translational
      modifications
      Phosphorylated on tyrosine, serine and threonine residues upon B-cell activation. Phosphorylation of tyrosine residues by Src-family kinases is an early and essential feature of the BCR signaling cascade. The phosphorylated tyrosines serve as docking sites for SH2-domain containing kinases, leading to their activation which in turn leads to phosphorylation of downstream targets. Phosphorylation of serine and threonine residues may prevent subsequent tyrosine phosphorylation.
    • Cellular localization
      Cell membrane. Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts.
    • Information by UniProt
    • Database links
    • Alternative names
      • B lymphocyte-specific MB1 protein antibody
      • B-cell antigen receptor complex-associated protein alpha chain antibody
      • CD 79a antibody
      • CD79a antibody
      • CD79a antigen (immunoglobulin-associated alpha) antibody
      • CD79A antigen antibody
      • CD79a molecule, immunoglobulin-associated alpha antibody
      • CD79A_HUMAN antibody
      • Ig alpha antibody
      • Ig-alpha antibody
      • IGA antibody
      • IgM-alpha antibody
      • Immunoglobulin-associated alpha antibody
      • Ly54 antibody
      • MB-1 membrane glycoprotein antibody
      • MB1 antibody
      • Membrane-bound immunoglobulin-associated protein antibody
      • Surface IgM-associated protein antibody
      see all

    References

    ab62650 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Ferret Tissue sections (Lymph nodes)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Commercial Target Retrieval Solution Low pH
    Permeabilization
    No
    Specification
    Lymph nodes
    Fixative
    Formaldehyde
    Username

    Jenni Harper

    Verified customer

    Submitted Sep 19 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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