Anti-CD79b antibody [CB3-1] (FITC) (ab25626)

Overview

  • Product nameAnti-CD79b antibody [CB3-1] (FITC)
    See all CD79b primary antibodies
  • Description
    Mouse monoclonal [CB3-1] to CD79b (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityThis antibody reacts with the beta chain of CD79.
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    The details of the immunogen for this antibody are not available.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone numberCB3-1
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 20µl for 106 cells. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

  • FunctionRequired in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation.
  • Tissue specificityB-cells.
  • Involvement in diseaseDefects in CD79B are the cause of agammaglobulinemia type 6 (AGM6) [MIM:612692]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life.
  • Sequence similaritiesContains 1 Ig-like V-type (immunoglobulin-like) domain.
    Contains 1 ITAM domain.
  • Post-translational
    modifications
    Phosphorylated on tyrosine upon B-cell activation.
  • Cellular localizationCell membrane. Following antigen binding, the BCR has been shown to translocate from detergent-soluble regions of the cell membrane to lipid rafts although signal transduction through the complex can also occur outside lipid rafts.
  • Information by UniProt
  • Database links
  • Alternative names
    • AGM6 antibody
    • B cell antigen receptor complex associated protein beta chain antibody
    • B cell specific glycoprotein B29 antibody
    • B-cell antigen receptor complex-associated protein beta chain antibody
    • B-cell-specific glycoprotein B29 antibody
    • B29 antibody
    • B29/Ig-beta/CD79b antibody
    • CD 79b antibody
    • CD79b antibody
    • CD79b antigen (immunoglobulin associated beta) antibody
    • CD79b antigen antibody
    • CD79b molecule antibody
    • CD79b molecule immunoglobulin associated beta antibody
    • CD79b protein antibody
    • CD79B_HUMAN antibody
    • Ig beta antibody
    • Ig-beta antibody
    • IGB antibody
    • Igbeta antibody
    • Immunoglobulin associated B29 antibody
    • Immunoglobulin associated B29 protein antibody
    • Immunoglobulin associated beta antibody
    • Immunoglobulin associated protein antibody
    • Immunoglobulin-associated B29 protein antibody
    • MGC108607 antibody
    see all

Anti-CD79b antibody [CB3-1] (FITC) images

  • Overlay histogram showing RAMOS cells stained with ab25626 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25626, 2µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 FITC (2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References for Anti-CD79b antibody [CB3-1] (FITC) (ab25626)

ab25626 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"